{"product_id":"anti-ret-receptor-extracellular-fitc-antibody-bha21300829","title":"Anti-Ret Receptor (extracellular)-FITC Antibody","description":"\u003ch2\u003eOverview\u003c\/h2\u003e \u003cp\u003eAnti-Ret Receptor (extracellular)-FITC Antibody is an antibody targeting Proto-oncogene tyrosine-protein kinase receptor Ret, Proto-oncogene c-Ret, Cadherin family member 12, CDHF12, PTC, RET51 Polyclonal raised in Rabbit (Fluorescein isothiocyanate (FITC)). This antibody is commonly used in FC, LCI to detect, localize, or compare expression of the target across samples.\u003c\/p\u003e  \u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e \u003cul\u003e   \u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e Proto-oncogene tyrosine-protein kinase receptor Ret, Proto-oncogene c-Ret, Cadherin family member 12, CDHF12, PTC, RET51 (also reported as Proto-oncogene tyrosine-protein kinase receptor Ret, Proto-oncogene c-Ret, Cadherin family member 12, CDHF12, PTC, RET51).\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eImmunogen\/epitope region:\u003c\/strong\u003e Extracellular, N-terminus.\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eHomology note:\u003c\/strong\u003e Mouse - identical; rat - 12\/13 amino acid residues identical (informative for cross-species interpretation).\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eSpecies reactivity (as provided):\u003c\/strong\u003e Human, Rat, Mouse.\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eLot quality control (as provided):\u003c\/strong\u003e Western blot analysis (unlabeled antibody, #ANT-025), and direct flow cytometry (labeled antibody)..\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003ePeptide confirmation:\u003c\/strong\u003e Confirmed by amino acid analysis and mass spectrometry.\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eBlocking peptide:\u003c\/strong\u003e Available for antigen preadsorption control where appropriate.\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eConjugate\/format:\u003c\/strong\u003e Fluorescein isothiocyanate (FITC) (may affect detection channel and background).\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eThese attributes help researchers interpret whether signal reflects the intended target in a given assay and sample context.\u003c\/p\u003e  \u003ch2\u003eBiological background\u003c\/h2\u003e \u003cp\u003eThe RET proto-oncogene on human chromosome 10q11.2, encodes a receptor tyrosine kinase (RTK) activated by members of the glial cell line-derived neurotrophic factor (GDNF) ligand family (GDNF, neurturin, artemin, and persephin) in conjunction with a ligand-specific coreceptor (GFRα1-4)1. RET belongs to the cadherin superfamily, and it has been suggested that the ret gene is the result of a recombination between a gene encoding a tyrosine kinase receptor and a gene encoding an ancestral cadherin at an early stage of evolution2. The extracellular domain of RET comprises, as observed for other tyrosine kinase receptors, a cysteine-rich domain, but also four cadherin-like domains3.RET signaling is crucial for the development of the enteric nervous system.\u003c\/p\u003e  \u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e \u003cul\u003e   \u003cli\u003eComparing target expression across perturbations, genotypes, or treatment conditions.\u003c\/li\u003e   \u003cli\u003eInterpreting localization shifts alongside pathway or phenotypic readouts.\u003c\/li\u003e   \u003cli\u003eUsing orthogonal controls (KO\/KD, peptide competition, isotype concepts) to support conclusions.\u003c\/li\u003e \u003c\/ul\u003e  \u003ch2\u003eCommon research applications\u003c\/h2\u003e \u003cul\u003e   \u003cli\u003eFlow cytometry (direct\/indirect): quantify target-positive populations and shifts in expression across subsets.\u003c\/li\u003e   \u003cli\u003eLive cell imaging (LCI): support extracellular-epitope detection on non-permeabilized cells when appropriate.\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eInterpretation typically benefits from comparing matched sample sets (e.g., treated vs control, WT vs KO\/KD) and using orthogonal readouts where feasible.\u003c\/p\u003e  \u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e \u003cul\u003e   \u003cli\u003eIsoforms and post-translational modifications can shift apparent molecular weight or epitope accessibility across samples.\u003c\/li\u003e   \u003cli\u003eCross-species signal may depend on epitope conservation; consult the provided homology note when selecting models.\u003c\/li\u003e   \u003cli\u003ePermeabilization, fixation, and antigen retrieval can change accessibility of intracellular vs extracellular epitopes.\u003c\/li\u003e   \u003cli\u003eConceptual control: antigen preadsorption (blocking peptide) can help assess signal dependence on the immunogen region.\u003c\/li\u003e   \u003cli\u003eProvided control suggestions: Negative control: RIC-001-F.\u003c\/li\u003e   \u003cli\u003eApplication notes: see product-specific dilution\/usage notes and control concepts provided in the dataset.\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003e\u003cstrong\u003eApplication abbreviations:\u003c\/strong\u003e CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot. \u003cstrong\u003eSpecies abbreviations:\u003c\/strong\u003e H- Human, M- Mouse, R- Rat.\u003c\/p\u003e \u003cp\u003e\u003cstrong\u003eRecommended controls:\u003c\/strong\u003e Blocking peptide: BLP-NT025; Negative control: RIC-001-F.\u003c\/p\u003e \u003c!-- Sources (internal): - Alomone Labs product page scientific background (as provided in this catalog row) - UniProt Knowledgebase (target-level reference) - NCBI Gene (target-level reference) - General antibody validation principles (KO\/KD, peptide competition, isotype control concepts) --\u003e","brand":"Alomone Labs","offers":[{"title":"15 mcl \/ 1","offer_id":53064842248557,"sku":"ANT-025-F-15MCL-1","price":597.0,"currency_code":"USD","in_stock":true},{"title":"50 mcl \/ 1","offer_id":53064936063341,"sku":"ANT-025-F-50MCL-1","price":797.0,"currency_code":"USD","in_stock":true},{"title":"50 mcl \/ 2","offer_id":53064936096109,"sku":"ANT-025-F-50MCL-2","price":1397.0,"currency_code":"USD","in_stock":true},{"title":"50 mcl \/ 5","offer_id":53064936128877,"sku":"ANT-025-F-50MCL-5","price":2743.0,"currency_code":"USD","in_stock":true},{"title":"50 mcl \/ 10","offer_id":53064936161645,"sku":"ANT-025-F-50MCL-10","price":3764.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/ANT-025-FC1.png?v=1772461034","url":"https:\/\/www.ebiohippo.com\/products\/anti-ret-receptor-extracellular-fitc-antibody-bha21300829","provider":"BioHippo","version":"1.0","type":"link"}