{"product_id":"anti-rtn4r-ngr1-nogor-antibody-m5-bha21405718","title":"Anti-RTN4R\/NgR1\/Nogor Antibody (M5)","description":"\u003cp\u003e\u003cstrong\u003eOverview\u003c\/strong\u003e\u003cbr\u003eAntibody against RTN4R\/NgR1\/Nogor (Reticulon-4 receptor) for research detection of protein expression and localization.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eTarget biology\u003c\/strong\u003e\u003cbr\u003eRTN4R encodes a receptor that transduces extracellular ligand binding into intracellular signaling, shaping cellular activation, differentiation or survival. Receptor expression and localization are frequently assessed in immunology and cancer biology, and receptor engagement often drives rapid phosphorylation and transcriptional responses.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eExpected localization\u003c\/strong\u003e\u003cbr\u003ePlasma membrane receptor; internalization and endosomal trafficking can occur upon ligand binding or antibody engagement.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eResearch context\u003c\/strong\u003e\u003cbr\u003eThis target is frequently studied in the context of \u003cem\u003eMolecular \u0026amp; Cellular Biology\u003c\/em\u003e research. Application suitability (e.g., ELISA, FCM, IF, IP, SPR, WB) is indicated in the specifications; conditions should be optimized by titration. Species reactivity information is provided in the specifications (reported: Human).\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eRecommended experimental notes\u003c\/strong\u003e\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003eFor Western blotting, optimize lysis conditions and include reducing\/non-reducing conditions as appropriate; titrate antibody to balance sensitivity and background.\u003c\/li\u003e\n\u003cli\u003eFor IF\/ICC, adjust permeabilization (e.g., mild detergent vs stronger conditions) based on expected localization; include secondary-only and isotype controls for background.\u003c\/li\u003e\n\u003cli\u003eFor IP, use gentle lysis to preserve complexes and include an IgG control; validate enrichment by immunoblot or orthogonal detection.\u003c\/li\u003e\n\u003cli\u003eFor ELISA, ensure capture\/detection antibody compatibility and run a standard curve; verify sample matrix effects with spike-and-recovery and dilution linearity.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eControls \u0026amp; interpretation\u003c\/strong\u003e\u003cbr\u003eWhere possible, confirm specificity using genetic perturbation (KO\/KD\/CRISPR), peptide competition (if applicable), or an orthogonal readout. Include a negative control sample expected to express low\/none of RTN4R\/NgR1\/Nogor, and a positive control sample known to express RTN4R\/NgR1\/Nogor.\u003c\/p\u003e\u003cp\u003e\u003cem\u003eFor research use only. Not for diagnostic procedures.\u003c\/em\u003e\u003c\/p\u003e","brand":"Biohippo Inc","offers":[{"title":"50 ug","offer_id":53036936298861,"sku":"HP096013-50UG","price":196.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53037545849197,"sku":"HP096013-100UG","price":326.0,"currency_code":"USD","in_stock":true},{"title":"1 mg","offer_id":53037545881965,"sku":"HP096013-1MG","price":1638.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/HP096013-SDS-PAGE-1.jpg?v=1771746131","url":"https:\/\/www.ebiohippo.com\/products\/anti-rtn4r-ngr1-nogor-antibody-m5-bha21405718","provider":"BioHippo","version":"1.0","type":"link"}