{"product_id":"anti-sars2-nsp5-3cl-pro-m-pro-polyclonal-antibody-bha21400502","title":"Anti-SARS2 NSP5\/3CL-PRO\/M Pro Polyclonal Antibody","description":"\u003ch3\u003eOverview\u003c\/h3\u003e\u003cp\u003eThis antibody enables detection of \u003cstrong\u003eSARS2 NSP5\/3CL-PRO\/M Pro\u003c\/strong\u003e (ORF1a polyprotein) \u003cspan style=\"color:#555\"\u003e(also known as ORF1ab)\u003c\/span\u003e in Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2) samples. It is designed to support ELISA, IHC, WB and related assays where specific target recognition is required.\u003c\/p\u003e\u003ch3\u003eTarget biology\u003c\/h3\u003e\u003cp\u003eORF1a polyprotein is a pathogen-derived antigen commonly used to study pathogen biology, host immune recognition, and assay development. Antibody-based detection supports tracking of antigen expression, processing, or localization in infected or antigen-presenting samples. Based on protein class and annotation keywords, it is most often associated with the \u003cstrong\u003evaries by cell type and isoform\u003c\/strong\u003e. This target is commonly used in \u003cstrong\u003eMolecular \u0026amp; Cellular Biology\u003c\/strong\u003e research.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eExpected localization:\u003c\/strong\u003e varies by cell type and isoform (may vary with cell type, activation state, and isoform).\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eResearch context:\u003c\/strong\u003e Frequently used in Molecular \u0026amp; Cellular Biology studies.\u003c\/p\u003e\u003ch3\u003eImmunogen\u003c\/h3\u003e\u003cp\u003e\u003cstrong\u003eImmunogen context:\u003c\/strong\u003e Raised against E. coli - derived recombinant SARS2 NSP5\/3CL-PRO\/M Pro (Ser2618-Gln2923).. Epitope accessibility can vary by assay format and sample treatment, so titration is recommended.\u003c\/p\u003e\u003ch3\u003eRecommended experimental notes\u003c\/h3\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTitrate for your system:\u003c\/strong\u003e Start with a mid-range working concentration and optimize for signal-to-background in your exact sample type.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eUse biological controls:\u003c\/strong\u003e Where possible, include a known positive sample and a target-low\/negative sample (or KO\/KD) to confirm specificity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eInterpret bands\/staining carefully:\u003c\/strong\u003e Many targets have isoforms, PTMs, or processing that can change apparent size or localization; corroborate with orthogonal readouts when available.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blot (WB):\u003c\/strong\u003e Optimize lysis\/denaturation conditions for epitope accessibility; include a positive lysate and consider reducing vs non-reducing conditions if the target is disulfide-rich or multimeric.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eIHC:\u003c\/strong\u003e Fixation and antigen retrieval can strongly influence staining. Start with a standard heat-induced retrieval condition and titrate antibody to minimize background; include known positive and negative tissues.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e For best sensitivity, optimize capture\/detection pairing and blocking conditions; test recombinant standard curves and matrix effects for your sample type.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cem\u003eFor research use only. Not for diagnostic procedures.\u003c\/em\u003e\u003c\/p\u003e","brand":"Biohippo Inc","offers":[{"title":"50 ug","offer_id":53036761579885,"sku":"VK635024-50UG","price":100.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53037341409645,"sku":"VK635024-100UG","price":160.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/www.ebiohippo.com\/products\/anti-sars2-nsp5-3cl-pro-m-pro-polyclonal-antibody-bha21400502","provider":"BioHippo","version":"1.0","type":"link"}