{"product_id":"anti-slc11a1-nramp1-extracellular-fitc-antibody-bha21300897","title":"Anti-SLC11A1\/NRAMP1 (extracellular)-FITC Antibody","description":"\u003ch2\u003eOverview\u003c\/h2\u003e \u003cp\u003eAnti-SLC11A1\/NRAMP1 (extracellular)-FITC Antibody is an antibody targeting Natural Resistance-Associated Macrophage Protein 1, LSH, Solute Carrier Family 11 Member 1 Polyclonal raised in Rabbit (Fluorescein isothiocyanate (FITC)). This antibody is commonly used in FC, LCI to detect, localize, or compare expression of the target across samples.\u003c\/p\u003e  \u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e \u003cul\u003e   \u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e Natural Resistance-Associated Macrophage Protein 1, LSH, Solute Carrier Family 11 Member 1 (also reported as Natural Resistance-Associated Macrophage Protein 1, LSH, Solute Carrier Family 11 Member 1).\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eImmunogen\/epitope region:\u003c\/strong\u003e 4th extracellular loop.\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eHomology note:\u003c\/strong\u003e Human, rat - 14\/15 amino acid residues identical; mouse - 13\/15 amino acid residues identical (informative for cross-species interpretation).\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eSpecies reactivity (as provided):\u003c\/strong\u003e Human, Rat, Mouse.\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eLot quality control (as provided):\u003c\/strong\u003e Western blot analysis (unlabeled antibody, #ANT-201), and direct flow cytometry (labeled antibody)..\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003ePeptide confirmation:\u003c\/strong\u003e Confirmed by amino acid analysis and mass spectrometry.\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eBlocking peptide:\u003c\/strong\u003e Available for antigen preadsorption control where appropriate.\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eConjugate\/format:\u003c\/strong\u003e Fluorescein isothiocyanate (FITC) (may affect detection channel and background).\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eThese attributes help researchers interpret whether signal reflects the intended target in a given assay and sample context.\u003c\/p\u003e  \u003ch2\u003eBiological background\u003c\/h2\u003e \u003cp\u003eSolute carrier family 11 member a1 (Slc11a1) also known as natural resistance-associated macrophage protein 1 (NRAMP1) is a proton\/divalent cation antiporter, known for its role in regulating the antimicrobial activity of macrophages1. The structure of Slc11a1 includes a common and conserved hydrophobic core of 10 transmembrane domains (TM) and either one or two non-conserved, highly hydrophobic TM domains4.Slc11a1 delivers divalent cations such as Fe2+, Zn2+, and Mn2+ from the cytosol to the late endosome\/lysosome acidic compartments where the Fenton reaction can use ferrous iron to generate toxic hydroxyl radicals2. Thus, providing one mechanism by which Slc11a1 could influence antimicrobial activity in macrophages.Transition of metal ions is essential and participates in numerous cellular functions such as regulation of transcription.\u003c\/p\u003e  \u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e \u003cul\u003e   \u003cli\u003eComparing target expression across perturbations, genotypes, or treatment conditions.\u003c\/li\u003e   \u003cli\u003eInterpreting localization shifts alongside pathway or phenotypic readouts.\u003c\/li\u003e   \u003cli\u003eUsing orthogonal controls (KO\/KD, peptide competition, isotype concepts) to support conclusions.\u003c\/li\u003e \u003c\/ul\u003e  \u003ch2\u003eCommon research applications\u003c\/h2\u003e \u003cul\u003e   \u003cli\u003eFlow cytometry (direct\/indirect): quantify target-positive populations and shifts in expression across subsets.\u003c\/li\u003e   \u003cli\u003eLive cell imaging (LCI): support extracellular-epitope detection on non-permeabilized cells when appropriate.\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eInterpretation typically benefits from comparing matched sample sets (e.g., treated vs control, WT vs KO\/KD) and using orthogonal readouts where feasible.\u003c\/p\u003e  \u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e \u003cul\u003e   \u003cli\u003eIsoforms and post-translational modifications can shift apparent molecular weight or epitope accessibility across samples.\u003c\/li\u003e   \u003cli\u003eCross-species signal may depend on epitope conservation; consult the provided homology note when selecting models.\u003c\/li\u003e   \u003cli\u003ePermeabilization, fixation, and antigen retrieval can change accessibility of intracellular vs extracellular epitopes.\u003c\/li\u003e   \u003cli\u003eConceptual control: antigen preadsorption (blocking peptide) can help assess signal dependence on the immunogen region.\u003c\/li\u003e   \u003cli\u003eProvided control suggestions: Negative control: RIC-001-F.\u003c\/li\u003e   \u003cli\u003eApplication notes: see product-specific dilution\/usage notes and control concepts provided in the dataset.\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003e\u003cstrong\u003eApplication abbreviations:\u003c\/strong\u003e CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot. \u003cstrong\u003eSpecies abbreviations:\u003c\/strong\u003e H- Human, M- Mouse, R- Rat.\u003c\/p\u003e \u003cp\u003e\u003cstrong\u003eRecommended controls:\u003c\/strong\u003e Blocking peptide: BLP-NT201; Negative control: RIC-001-F.\u003c\/p\u003e \u003c!-- Sources (internal): - Alomone Labs product page scientific background (as provided in this catalog row) - UniProt Knowledgebase (target-level reference) - NCBI Gene (target-level reference) - General antibody validation principles (KO\/KD, peptide competition, isotype control concepts) --\u003e","brand":"Alomone Labs","offers":[{"title":"15 mcl \/ 1","offer_id":53064845951341,"sku":"ANT-201-F-15MCL-1","price":597.0,"currency_code":"USD","in_stock":true},{"title":"50 mcl \/ 1","offer_id":53064945434989,"sku":"ANT-201-F-50MCL-1","price":797.0,"currency_code":"USD","in_stock":true},{"title":"50 mcl \/ 2","offer_id":53064945467757,"sku":"ANT-201-F-50MCL-2","price":1397.0,"currency_code":"USD","in_stock":true},{"title":"50 mcl \/ 5","offer_id":53064945500525,"sku":"ANT-201-F-50MCL-5","price":2743.0,"currency_code":"USD","in_stock":true},{"title":"50 mcl \/ 10","offer_id":53064945533293,"sku":"ANT-201-F-50MCL-10","price":3764.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/ANT-201-F-J774-2.png?v=1772461042","url":"https:\/\/www.ebiohippo.com\/products\/anti-slc11a1-nramp1-extracellular-fitc-antibody-bha21300897","provider":"BioHippo","version":"1.0","type":"link"}