{"product_id":"anti-smurf1-antibody-l12-30-bha21406009","title":"Anti-Smurf1 Antibody (L12\/30)","description":"\u003cp\u003e\u003cstrong\u003eOverview\u003c\/strong\u003e\u003cbr\u003eAntibody against Smurf1 (E3 ubiquitin-protein ligase SMURF1) for research detection of protein expression and localization.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eTarget biology\u003c\/strong\u003e\u003cbr\u003eE3 ubiquitin-protein ligase SMURF1 is a ligase that catalyzes formation of covalent bonds (often ATP-dependent) and can participate in processes such as ubiquitin-like conjugation, DNA repair or metabolic pathway assembly depending on the ligase family. Ligases are commonly investigated for roles in protein turnover and cellular stress responses.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eExpected localization\u003c\/strong\u003e\u003cbr\u003eLocalization varies by ligase family; many are cytosolic\/nuclear, with some organellar forms.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eResearch context\u003c\/strong\u003e\u003cbr\u003eThis target is frequently studied in the context of \u003cem\u003eProtein Homeostasis \u0026amp; Degradation\u003c\/em\u003e research. Application suitability (e.g., IHC, WB) is indicated in the specifications; conditions should be optimized by titration. Species reactivity information is provided in the specifications (reported: Human, Mouse).\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eRecommended experimental notes\u003c\/strong\u003e\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003eFor Western blotting, optimize lysis conditions and include reducing\/non-reducing conditions as appropriate; titrate antibody to balance sensitivity and background.\u003c\/li\u003e\n\u003cli\u003eFor IHC, fixation and antigen retrieval strongly influence epitope accessibility; test citrate- vs EDTA-based retrieval and include matched positive\/negative tissues.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eControls \u0026amp; interpretation\u003c\/strong\u003e\u003cbr\u003eWhere possible, confirm specificity using genetic perturbation (KO\/KD\/CRISPR), peptide competition (if applicable), or an orthogonal readout. Include a negative control sample expected to express low\/none of Smurf1, and a positive control sample known to express Smurf1.\u003c\/p\u003e\u003cp\u003e\u003cem\u003eFor research use only. Not for diagnostic procedures.\u003c\/em\u003e\u003c\/p\u003e","brand":"Biohippo Inc","offers":[{"title":"50 ug","offer_id":53036946882925,"sku":"HA278013-50UG","price":196.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53037565542765,"sku":"HA278013-100UG","price":326.0,"currency_code":"USD","in_stock":true},{"title":"1 mg","offer_id":53037565575533,"sku":"HA278013-1MG","price":1638.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/HA278013-SDS-PAGE-1.jpg?v=1771746273","url":"https:\/\/www.ebiohippo.com\/products\/anti-smurf1-antibody-l12-30-bha21406009","provider":"BioHippo","version":"1.0","type":"link"}