{"product_id":"anti-snap25-polyclonal-antibody-bha21404051","title":"Anti-SNAP25 Polyclonal Antibody","description":"\u003cp\u003e\u003cstrong\u003eOverview\u003c\/strong\u003e\u003cbr\u003eAntibody against SNAP25 (Synaptosomal-associated 25 kDa protein) for research detection of protein expression and localization.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eTarget biology\u003c\/strong\u003e\u003cbr\u003eSNAP25 is a core SNARE complex component required for calcium-triggered synaptic vesicle exocytosis and neurotransmitter release. It is a widely used presynaptic marker for neuronal terminals and is studied in synaptic function, plasticity and neurodevelopmental disease models.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eExpected localization\u003c\/strong\u003e\u003cbr\u003eEnriched at presynaptic membranes and neuronal processes; associates with plasma membrane via palmitoylation.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eResearch context\u003c\/strong\u003e\u003cbr\u003eThis target is frequently studied in the context of \u003cem\u003eMolecular \u0026amp; Cellular Biology\u003c\/em\u003e research. Application suitability (e.g., ELISA, IHC, WB) is indicated in the specifications; conditions should be optimized by titration. Species reactivity information is provided in the specifications (reported: Human, Mouse, Rat).\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eRecommended experimental notes\u003c\/strong\u003e\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003eFor Western blotting, optimize lysis conditions and include reducing\/non-reducing conditions as appropriate; titrate antibody to balance sensitivity and background.\u003c\/li\u003e\n\u003cli\u003eFor IHC, fixation and antigen retrieval strongly influence epitope accessibility; test citrate- vs EDTA-based retrieval and include matched positive\/negative tissues.\u003c\/li\u003e\n\u003cli\u003eFor ELISA, ensure capture\/detection antibody compatibility and run a standard curve; verify sample matrix effects with spike-and-recovery and dilution linearity.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eControls \u0026amp; interpretation\u003c\/strong\u003e\u003cbr\u003eWhere possible, confirm specificity using genetic perturbation (KO\/KD\/CRISPR), peptide competition (if applicable), or an orthogonal readout. Include a negative control sample expected to express low\/none of SNAP25, and a positive control sample known to express SNAP25.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eImmunogen context\u003c\/strong\u003e\u003cbr\u003eGenerated using an immunogen derived from E. coli - derived recombinant Human SNAP25 (Met1-Gly206).. When interpreting results, note that epitope accessibility can vary with denaturation, fixation, or post‑translational modifications; empirical optimization is recommended.\u003c\/p\u003e\u003cp\u003e\u003cem\u003eFor research use only. Not for diagnostic procedures.\u003c\/em\u003e\u003c\/p\u003e","brand":"Biohippo Inc","offers":[{"title":"50 ug","offer_id":53036881609069,"sku":"HX087014-50UG","price":100.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53037461111149,"sku":"HX087014-100UG","price":160.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/HX087014-abinscience-WB-1.jpg?v=1771745111","url":"https:\/\/www.ebiohippo.com\/products\/anti-snap25-polyclonal-antibody-bha21404051","provider":"BioHippo","version":"1.0","type":"link"}