{"product_id":"anti-tf-ag-antibody-nm-tf1-pe-bha21418521","title":"Anti-TF-Ag Antibody (NM-TF1), PE","description":"\u003cp\u003e\u003cstrong\u003eOverview\u003c\/strong\u003e\u003cbr\u003e\nNormal Guinea pig IgG Isotype Control Antibody is designed to match primary antibody format for background estimation. Commonly used in FCM, IHC, IF, Isotype Control applications. Typical workflows include Select staining controls in cells\/tissues (isotype\/KD\/KO), Detect target localization by IF\/ICC in cultured cells, Profile target expression by IHC in FFPE tissue sections, Optimize antibody dilution for staining in cells\/tissues using a reference sample, QC lot-to-lot by comparing signal in a reference lysate or cell line. Relevant research areas: Molecular \u0026amp; Cellular Biology.\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003eReagent details:\u003c\/strong\u003e Host: Guinea pig; Clonality: Polyclonal; Isotype: IgG; Purification: Protein A\/G purified from cell culture supernatant.; Format: Liquid.\u003c\/p\u003e\n\n\u003ch3\u003eIsotype control rationale\u003c\/h3\u003e\n\u003cp\u003eIsotype control antibodies are matched to the primary antibody’s species and isotype, but lack specificity for a cellular antigen. They are used to estimate non‑specific binding and background signal in immunoassays. In flow cytometry and immunofluorescence, isotype controls can help identify staining driven by Fc receptor interactions, sticky epitopes, or fluorophore\/label-associated background rather than true antigen recognition. This reagent is characterized by its host species (Guinea pig), isotype (IgG), which should match your primary antibody format as closely as possible.\u003c\/p\u003e\n\n\u003ch3\u003eHow this reagent helps\u003c\/h3\u003e\n\u003cp\u003eUse this isotype control to estimate non-specific background under matched staining conditions. For best performance, interpret signal in the context of appropriate controls and orthogonal validation when available.\u003c\/p\u003e\n\n\u003ch3\u003eRecommended experimental notes\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eTitrate the antibody\/reagent to optimize signal-to-noise for your specific sample type and detection system.\u003c\/li\u003e\n\u003cli\u003eRun the isotype control at the same concentration, incubation time, and staining conditions as the test antibody.\u003c\/li\u003e\n\u003cli\u003eFor flow cytometry, consider Fc blocking and use an FMO (fluorescence minus one) control to support gating decisions.\u003c\/li\u003e\n\u003cli\u003eInclude appropriate controls (e.g., unstained, secondary-only where relevant, and biological positives\/negatives).\u003c\/li\u003e\n\u003cli\u003eFor IHC, optimize fixation and antigen retrieval (buffer\/pH\/heat) and include isotype\/secondary-only controls to assess background.\u003c\/li\u003e\n\u003cli\u003eFor IF\/ICC, test fixation (PFA vs methanol) and permeabilization conditions to preserve epitope accessibility while minimizing background.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch3\u003eStorage\u003c\/h3\u003e\u003cp\u003eUse a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. 0.01M PBS, pH 7.4.\u003c\/p\u003e\n\n\u003cp\u003e\u003cem\u003eFor research use only. Not for diagnostic procedures.\u003c\/em\u003e\u003c\/p\u003e","brand":"Biohippo Inc","offers":[{"title":"100 T","offer_id":53037270761837,"sku":"YA245237-100T","price":482.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/www.ebiohippo.com\/products\/anti-tf-ag-antibody-nm-tf1-pe-bha21418521","provider":"BioHippo","version":"1.0","type":"link"}