{"product_id":"anti-ube2n-polyclonal-antibody-bha21416806","title":"Anti-UBE2N Polyclonal Antibody","description":"\u003cp\u003e\u003cstrong\u003eOverview\u003c\/strong\u003e\u003cbr\u003e\nAnti-UBE2N Polyclonal Antibody targets \u003cstrong\u003eUBE2N\u003c\/strong\u003e (Ubiquitin-protein ligase N). Frequently used across ELISA, IHC, WB workflows, depending on experimental design and sample type. This antibody is suited for workflows commonly used in Protein Homeostasis \u0026amp; Degradation research.\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003eAlso known as:\u003c\/strong\u003e Ubiquitin-protein ligase N, Ubiquitin carrier protein N, Bendless-like ubiquitin-conjugating enzyme, Ubiquitin-conjugating enzyme E2 N, E2 ubiquitin-conjugating enzyme N, 2.3.2.23, UbcH13, Ubc13, BLU.\u003c\/p\u003e\n\n\u003ch3\u003eTarget biology\u003c\/h3\u003e\n\u003cp\u003eUBE2N (Ubiquitin-protein ligase N) encodes an E3 ubiquitin ligase component that tags substrates with ubiquitin to regulate stability, trafficking, or signaling. Ubiquitin pathway proteins are commonly evaluated in proteostasis and signal turnover mechanisms. Common alternative names and symbols used in the literature include: Ubiquitin carrier protein N, Bendless-like ubiquitin-conjugating enzyme, Ubiquitin-conjugating enzyme E2 N, E2 ubiquitin-conjugating enzyme N, 2.3.2.23, UbcH13, Ubc13, BLU. Based on the annotated name\/class, the target is most often associated with context-dependent localization, though this can vary by model system.\u003c\/p\u003e\n\n\u003ch3\u003eHow this antibody helps\u003c\/h3\u003e\n\u003cp\u003eAs a polyclonal reagent, this antibody can provide strong sensitivity through recognition of multiple epitopes on the target. For best performance, interpret signal in the context of appropriate biological controls and orthogonal readouts when available.\u003c\/p\u003e\n\n\u003ch3\u003eAntigen \/ immunogen context\u003c\/h3\u003e\u003cp\u003eE. coli - derived recombinant human UBE2N (Met1-Ile152).\u003c\/p\u003e\n\n\u003ch3\u003eRecommended experimental notes\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eTitrate the antibody to optimize signal-to-noise for your specific sample type and detection system.\u003c\/li\u003e\n\u003cli\u003eUse appropriate positive and negative controls (e.g., target-high vs target-low samples, KO\/KD where available).\u003c\/li\u003e\n\u003cli\u003eFor Western blotting, use fresh protease (and phosphatase, if relevant) inhibitors and confirm the primary band at the expected molecular weight; additional bands may reflect isoforms or processing.\u003c\/li\u003e\n\u003cli\u003eFor IHC, optimize fixation and antigen retrieval (buffer\/pH\/heat) and include an isotype\/secondary-only control to assess background.\u003c\/li\u003e\n\u003cli\u003eFor ELISA, optimize capture\/detection pairing and run a standard curve in matrix-matched diluent when possible.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch3\u003eAdditional notes\u003c\/h3\u003e\u003cp\u003eFor research use only\u003c\/p\u003e\n\n\u003cp\u003e\u003cem\u003eFor research use only. Not for diagnostic procedures.\u003c\/em\u003e\u003c\/p\u003e","brand":"Biohippo Inc","offers":[{"title":"50 ug","offer_id":53037214237037,"sku":"HB321014-50UG","price":100.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53037853475181,"sku":"HB321014-100UG","price":160.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/HB321014-abinscience-WB-1.jpg?v=1771749707","url":"https:\/\/www.ebiohippo.com\/products\/anti-ube2n-polyclonal-antibody-bha21416806","provider":"BioHippo","version":"1.0","type":"link"}