{"product_id":"anti-ube3a-monoclonal-antibody-bha21008932","title":"Anti-UBE3A Monoclonal Antibody","description":"\u003ch2\u003eOverview\u003c\/h2\u003e \u003cp\u003eThis product is an anti-UBE3A antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone ADBA-21; isotype Rabbit IgG; reactivity: Human,Mouse,Rat. Reported application contexts include WB (as provided in the source record). Boster Bio Anti-UBE3A Monoclonal Antibody catalog # M00582. Tested in WB application. This antibody reacts with Human, Mouse, Rat.\u003c\/p\u003e \u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e \u003cul\u003e \u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e UBE3A (Interferon regulatory factor 1).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody format:\u003c\/strong\u003e Monoclonal; clone ADBA-21; isotype Rabbit IgG.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHost:\u003c\/strong\u003e Rabbit.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSpecies reactivity:\u003c\/strong\u003e Human,Mouse,Rat (confirm in your model system with appropriate controls).\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eThis description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.\u003c\/p\u003e \u003ch2\u003eBiological background\u003c\/h2\u003e \u003cp\u003eUBE3A (protein: T-cell surface glycoprotein CD4) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Transcriptional regulator which displays a remarkable functional diversity in the regulation of cellular responses. These include the regulation of IFN and IFN-inducible genes, host response to viral and bacterial infections, regulation of many genes expressed during hematopoiesis, inflammation, immune responses and cell proliferation and differentiation, regulation of the cell cycle and induction of growth arrest and programmed cell death following DNA damage. Stimulates both innate and acquired immune responses through the activation of specific target genes and can act as a transcriptional activator and repressor regulating target genes by binding to an interferon- stimulated response element (ISRE) in their promoters. Its target genes for transcriptional activation activity include: genes involved in anti-viral response, such as IFN-alpha\/beta, DDX58\/RIG-I, TNFSF10\/TRAIL, OAS1\/2, PIAS1\/GBP, EIF2AK2\/PKR and RSAD2\/viperin; antibacterial response, such as NOS2\/INOS; anti- proliferative response, such as p53\/TP53, LOX and CDKN1A; apoptosis, such as BBC3\/PUMA, CASP1, CASP7 and CASP8; immune response, such as IL7, IL12A\/B and IL15, PTGS2\/COX2 and CYBB; DNA damage responses and DNA repair, such as POLQ\/POLH; MHC class I expression, such as TAP1, PSMB9\/LMP2, PSME1\/PA28A, PSME2\/PA28B and B2M and MHC class II expression, such as CIITA. Represses genes involved in anti-proliferative response, such as BIRC5\/survivin, CCNB1, CCNE1, CDK1, CDK2 and CDK4 and in immune response, such as FOXP3, IL4, ANXA2 and TLR4. Stimulates p53\/TP53-dependent transcription through enhanced recruitment of EP300 leading to increased acetylation of p53\/TP53. Plays an important role in immune response ly affecting NK maturation and activity, macrophage production of IL12, Th1 development and maturation of CD8+ T-cells. Also implicated in the differentiation and maturation of dendritic cells and in the suppression of regulatory T (Treg) cells development. Acts as a tumor suppressor and plays a role not only in antagonism of tumor cell growth but also in stimulating an immune response against tumor cells. . Reported cellular localization context: Nucleus. Cytoplasm. MYD88-associated IRF1 migrates into the nucleus more efficiently than non-MYD88- associated IRF1. Tissue expression notes (as provided): Heart, placenta, skeletal muscle, kidney, lung and pancreas. .\u003c\/p\u003e \u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e \u003cul\u003e \u003cli\u003eResearch context keywords from the source record include: Cell Biology,Chromatin Modifying Enzymes,Co-Activators\/Co-Repressors,Epigenetics and Nuclear Signaling,Neurodegenerative Disease,Neurology Process,Neuroscience,Nuclear Receptors,Nuclear Signaling Pathways,Proteasome \/ Ubiquitin,Proteolysis\/Ubiquitin,Ubiquitin E3 Enzymes.\u003c\/li\u003e\n\u003cli\u003eCurrent studies often focus on connecting target abundance\/localization to pathway perturbations across models, tissues, and cell states.\u003c\/li\u003e\n\u003cli\u003eQuantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.\u003c\/li\u003e \u003c\/ul\u003e \u003ch2\u003eCommon research applications\u003c\/h2\u003e \u003cul\u003e \u003cli\u003e\n\u003cstrong\u003eWestern blotting (WB):\u003c\/strong\u003e assess relative target abundance across samples, treatments, or time-points.\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eWorkflow ideas (metafield): Validate UBE3A antibody specificity using KO\/KD control samples (WB\/IF\/IHC as appropriate), Detect UBE3A expression by Western blot in cell or tissue lysates, Compare relative UBE3A levels across experimental conditions (dose\/time-course) using antibody-based readouts\u003c\/p\u003e \u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e \u003cul\u003e \u003cli\u003eConsider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.\u003c\/li\u003e\n\u003cli\u003eApparent molecular weight may vary by sample type and processing (observed MW: 47 kDa; calculated MW: 36502 MW).\u003c\/li\u003e\n\u003cli\u003eControl concepts: include appropriate negative controls (e.g., isotype, KO\/KD samples) and orthogonal validation when feasible.\u003c\/li\u003e \u003c\/ul\u003e \u003ch2\u003eAdditional product details (from the source record)\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eMolecular weight (observed):\u003c\/strong\u003e 47 kDa\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCellular localization (provided):\u003c\/strong\u003e Nucleus. Cytoplasm. MYD88-associated IRF1 migrates into the nucleus more efficiently than non-MYD88- associated IRF1.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTissue details (provided):\u003c\/strong\u003e Heart, placenta, skeletal muscle, kidney, lung and pancreas. .\u003c\/li\u003e\n\u003c\/ul\u003e \u003c!-- Sources (internal): - Antibodies — a laboratory manual overview — Cold Spring Harbor Protocols — https:\/\/cshprotocols.cshlp.org\/ - UniProt Knowledgebase — UniProt — https:\/\/www.uniprot.org\/ - NCBI Gene — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/ - Antibody validation and reproducibility — Nature methods (collections) — https:\/\/www.nature.com\/collections\/ - Immunohistochemistry\/Immunofluorescence basics — NIH \/ NCBI Bookshelf — https:\/\/www.ncbi.nlm.nih.gov\/books\/ --\u003e","brand":"Boster Bio","offers":[{"title":"100 uL\/vial \/ Unconjugated","offer_id":53071954575725,"sku":"M00582","price":370.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/m00582-wb.jpg?v=1772618797","url":"https:\/\/www.ebiohippo.com\/products\/anti-ube3a-monoclonal-antibody-bha21008932","provider":"BioHippo","version":"1.0","type":"link"}