{"product_id":"anti-urelumab-elisa-kit-bhe21400438","title":"Anti-Urelumab ELISA Kit","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}\u003c\/style\u003e\n\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eThe Anti-Urelumab ELISA Kit is a sandwich ELISA kit for quantitative measurement of anti-urelumab antibodies in serum, plasma and other biological fluid samples. The 96-well microplate is pre-coated, and the colorimetric signal is read at 450 nm.\u003c\/p\u003e\n\u003cp\u003eIn this sandwich format, anti-urelumab antibodies in the sample bind in the pre-coated wells and are then detected with an enzyme-labeled reagent, so the optical density rises with the antibody level. Results are calculated from the standard curve run on the same plate.\u003c\/p\u003e\n\u003cp\u003eValues are expressed relative to the antibody standard supplied with the kit.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eAssay Type\u003c\/th\u003e\n\u003ctd\u003eSandwich (Quantitative)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDetection Method\u003c\/th\u003e\n\u003ctd\u003eColorimetric (OD 450 nm)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSample Type\u003c\/th\u003e\n\u003ctd\u003eSerum, Plasma, Other Biological Fluid Samples\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSensitivity\u003c\/th\u003e\n\u003ctd\u003e0.31 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDetection Range\u003c\/th\u003e\n\u003ctd\u003e1.56 - 100 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eRecovery\u003c\/th\u003e\n\u003ctd\u003e80-120%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePrecision\u003c\/th\u003e\n\u003ctd\u003eIntra-assay CV \u0026lt;15%; Inter-assay CV \u0026lt;15%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eKit Size\u003c\/th\u003e\n\u003ctd\u003e96 T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShipping\u003c\/th\u003e\n\u003ctd\u003e2-8 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth style=\"width:auto\"\u003eComponent\u003c\/th\u003e\n\u003cth style=\"width:auto\"\u003eQuantity\u003c\/th\u003e\n\u003cth style=\"width:auto\"\u003eStorage\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePre-coated Microplate\u003c\/td\u003e\n\u003ctd\u003e1 plate\u003c\/td\u003e\n\u003ctd\u003e-20 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStandard\u003c\/td\u003e\n\u003ctd\u003e2 bottles\u003c\/td\u003e\n\u003ctd\u003e-20 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDetection A\u003c\/td\u003e\n\u003ctd\u003e1 vial\u003c\/td\u003e\n\u003ctd\u003e-20 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDetection B\u003c\/td\u003e\n\u003ctd\u003e1 vial\u003c\/td\u003e\n\u003ctd\u003e-20 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStandard Diluent\u003c\/td\u003e\n\u003ctd\u003e1 bottle\u003c\/td\u003e\n\u003ctd\u003e4 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAssay Diluent\u003c\/td\u003e\n\u003ctd\u003e1 bottle\u003c\/td\u003e\n\u003ctd\u003e4 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e20× Wash Buffer\u003c\/td\u003e\n\u003ctd\u003e1 bottle\u003c\/td\u003e\n\u003ctd\u003e4 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eColor Reagent\u003c\/td\u003e\n\u003ctd\u003e1 bottle\u003c\/td\u003e\n\u003ctd\u003e4 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStop Solution\u003c\/td\u003e\n\u003ctd\u003e1 bottle\u003c\/td\u003e\n\u003ctd\u003e4 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePlate Sealers\u003c\/td\u003e\n\u003ctd\u003e4 strips\u003c\/td\u003e\n\u003ctd\u003eRoom temperature\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eAssay Procedure (Summary)\u003c\/h2\u003e\n\u003cp\u003eBring all reagents to room temperature and prepare working solutions and the standard series before starting.\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eAdd 100 µL standard or sample; incubate 1 h at 37 °C.\u003c\/li\u003e\n\u003cli\u003eWash 3 times with 300 µL wash buffer per well.\u003c\/li\u003e\n\u003cli\u003eAdd 100 µL Detection A working solution; incubate 1 h at 37 °C.\u003c\/li\u003e\n\u003cli\u003eWash 3 times with 300 µL wash buffer per well.\u003c\/li\u003e\n\u003cli\u003eAdd 100 µL Detection B working solution; incubate 30 min at 37 °C.\u003c\/li\u003e\n\u003cli\u003eWash 5 times with 300 µL wash buffer per well.\u003c\/li\u003e\n\u003cli\u003eAdd 100 µL Color Reagent; incubate 15 min at 37 °C, protected from light.\u003c\/li\u003e\n\u003cli\u003eAdd 50 µL stop solution; the color turns from blue to yellow.\u003c\/li\u003e\n\u003cli\u003eRead the OD at 450 nm within 10 min (wavelength correction at 630 nm or 620 nm).\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003eTotal incubation time is about 2 h 45 min, plus washing steps. Required but not supplied: a microplate reader for 450 nm (correction at 630 nm or 620 nm), pipettes and tips, deionized or distilled water, a 500 mL graduated cylinder, a squirt bottle, manifold dispenser or plate washer, and tubes for standard dilutions.\u003c\/p\u003e\n\u003ch2\u003eSample Preparation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eSerum: use a serum separator tube, let the sample clot for 30 minutes at room temperature, and centrifuge for 15 minutes at 1000 × g.\u003c\/li\u003e\n\u003cli\u003ePlasma: collect with EDTA, heparin or citrate as the anticoagulant and centrifuge for 15 minutes at 1000 × g within 30 minutes of collection.\u003c\/li\u003e\n\u003cli\u003eAssay samples promptly or store aliquots at -20 °C or below; avoid repeated freeze-thaw cycles.\u003c\/li\u003e\n\u003cli\u003eSerum and plasma samples: a 10-fold starting dilution in Standard Diluent is suggested; adjust it so readings fall within the standard curve.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eStorage \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003eShips at 2-8 °C. For longer storage of unopened kits, keep the pre-coated microplate, standard, Detection A and Detection B at -20 °C and the other liquid reagents at 4 °C. Bring reagents to room temperature before use and do not mix components from different kit lots.\u003c\/div\u003e\n\u003cp\u003eFor Research Use Only. Not for use in diagnostic procedures.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"AtaGenix Laboratories","offers":[{"title":"96 T","offer_id":67980402262381,"sku":"AC336018-96T","price":1498.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/abinScience_Kit_Placeholder_Image_39d0572a-2a87-4d27-9239-67af446f5b60.jpg?v=1794521013","url":"https:\/\/www.ebiohippo.com\/products\/anti-urelumab-elisa-kit-bhe21400438","provider":"BioHippo","version":"1.0","type":"link"}