{"product_id":"anti-urelumab-neutralizing-antibody-elisa-kit-bhe21400439","title":"Anti-Urelumab Neutralizing Antibody ELISA Kit","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}\u003c\/style\u003e\n\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eThe Anti-Urelumab Neutralizing Antibody ELISA Kit is a competitive ELISA kit for quantitative measurement of neutralizing anti-urelumab antibodies in serum, plasma and other biological fluid samples. The 96-well microplate is pre-coated, and the colorimetric signal is read at 450 nm.\u003c\/p\u003e\n\u003cp\u003eIn this competitive format, neutralizing anti-urelumab antibodies in the sample block the binding of urelumab to its target in the coated wells, so the optical density falls as the neutralizing antibody level rises. Results are calculated from the standard curve run on the same plate.\u003c\/p\u003e\n\u003cp\u003eValues are expressed relative to the antibody standard supplied with the kit.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eAssay Type\u003c\/th\u003e\n\u003ctd\u003eCompetitive (Quantitative)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDetection Method\u003c\/th\u003e\n\u003ctd\u003eColorimetric (OD 450 nm)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSample Type\u003c\/th\u003e\n\u003ctd\u003eSerum, Plasma, Other Biological Fluid Samples\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSensitivity\u003c\/th\u003e\n\u003ctd\u003e6.95 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDetection Range\u003c\/th\u003e\n\u003ctd\u003e31.25 - 2,000 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eRecovery\u003c\/th\u003e\n\u003ctd\u003e80-120%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePrecision\u003c\/th\u003e\n\u003ctd\u003eIntra-assay CV \u0026lt;15%; Inter-assay CV \u0026lt;15%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eKit Size\u003c\/th\u003e\n\u003ctd\u003e96 T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShipping\u003c\/th\u003e\n\u003ctd\u003e2-8 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth style=\"width:auto\"\u003eComponent\u003c\/th\u003e\n\u003cth style=\"width:auto\"\u003eQuantity\u003c\/th\u003e\n\u003cth style=\"width:auto\"\u003eStorage\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePre-coated Microplate\u003c\/td\u003e\n\u003ctd\u003e1 plate\u003c\/td\u003e\n\u003ctd\u003e-20 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStandard\u003c\/td\u003e\n\u003ctd\u003e2 bottles\u003c\/td\u003e\n\u003ctd\u003e-20 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDetection A\u003c\/td\u003e\n\u003ctd\u003e1 vial\u003c\/td\u003e\n\u003ctd\u003e-20 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDetection B\u003c\/td\u003e\n\u003ctd\u003e1 vial\u003c\/td\u003e\n\u003ctd\u003e-20 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStandard Diluent\u003c\/td\u003e\n\u003ctd\u003e1 bottle\u003c\/td\u003e\n\u003ctd\u003e4 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAssay Diluent\u003c\/td\u003e\n\u003ctd\u003e1 bottle\u003c\/td\u003e\n\u003ctd\u003e4 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e20× Wash Buffer\u003c\/td\u003e\n\u003ctd\u003e1 bottle\u003c\/td\u003e\n\u003ctd\u003e4 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eColor Reagent\u003c\/td\u003e\n\u003ctd\u003e1 bottle\u003c\/td\u003e\n\u003ctd\u003e4 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStop Solution\u003c\/td\u003e\n\u003ctd\u003e1 bottle\u003c\/td\u003e\n\u003ctd\u003e4 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePlate Sealers\u003c\/td\u003e\n\u003ctd\u003e4 strips\u003c\/td\u003e\n\u003ctd\u003eRoom temperature\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eAssay Procedure (Summary)\u003c\/h2\u003e\n\u003cp\u003eBring all reagents to room temperature and prepare working solutions and the standard series before starting.\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eAdd 100 µL standard or sample premixed with Detection A working solution (50 µL + 50 µL); incubate 1 h at 37 °C.\u003c\/li\u003e\n\u003cli\u003eWash 3 times with 300 µL wash buffer per well.\u003c\/li\u003e\n\u003cli\u003eAdd 100 µL Detection B working solution; incubate 30 min at 37 °C.\u003c\/li\u003e\n\u003cli\u003eWash 5 times with 300 µL wash buffer per well.\u003c\/li\u003e\n\u003cli\u003eAdd 100 µL Color Reagent; incubate 15 min at 37 °C, protected from light.\u003c\/li\u003e\n\u003cli\u003eAdd 50 µL stop solution; the color turns from blue to yellow.\u003c\/li\u003e\n\u003cli\u003eRead the OD at 450 nm within 10 min (wavelength correction at 630 nm or 620 nm).\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003eTotal incubation time is about 1 h 45 min, plus washing steps. Required but not supplied: a microplate reader for 450 nm (correction at 630 nm or 620 nm), pipettes and tips, deionized or distilled water, a 500 mL graduated cylinder, a squirt bottle, manifold dispenser or plate washer, and tubes for standard dilutions.\u003c\/p\u003e\n\u003ch2\u003eSample Preparation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eSerum: use a serum separator tube, let the sample clot for 30 minutes at room temperature, and centrifuge for 15 minutes at 1000 × g.\u003c\/li\u003e\n\u003cli\u003ePlasma: collect with EDTA, heparin or citrate as the anticoagulant and centrifuge for 15 minutes at 1000 × g within 30 minutes of collection.\u003c\/li\u003e\n\u003cli\u003eAssay samples promptly or store aliquots at -20 °C or below; avoid repeated freeze-thaw cycles.\u003c\/li\u003e\n\u003cli\u003eSerum and plasma samples: a 10-fold starting dilution in Standard Diluent is suggested; adjust it so readings fall within the standard curve.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eStorage \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003eShips at 2-8 °C. For longer storage of unopened kits, keep the pre-coated microplate, standard, Detection A and Detection B at -20 °C and the other liquid reagents at 4 °C. Bring reagents to room temperature before use and do not mix components from different kit lots.\u003c\/div\u003e\n\u003cp\u003eFor Research Use Only. Not for use in diagnostic procedures.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"AtaGenix Laboratories","offers":[{"title":"96 T","offer_id":67980403278189,"sku":"AC336028-96T","price":2298.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/abinScience_Kit_Placeholder_Image_c2246e51-3919-431b-bb3e-af290bc87912.jpg?v=1794521011","url":"https:\/\/www.ebiohippo.com\/products\/anti-urelumab-neutralizing-antibody-elisa-kit-bhe21400439","provider":"BioHippo","version":"1.0","type":"link"}