{"product_id":"anti-vapb-antibody-picoband-bha21001701","title":"Anti-VAPB Antibody Picoband®","description":"\u003ch2\u003eOverview\u003c\/h2\u003e \u003cp\u003eThis antibody is intended for detection of \u003cstrong\u003eVAPB\u003c\/strong\u003e in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality\/clone information, and detection modality.\u003c\/p\u003e \u003cp\u003e\u003cem\u003eVendor notes:\u003c\/em\u003e Boster Bio Anti-VAPB Antibody Picoband® catalog # A01372. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.\u003c\/p\u003e \u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e \u003cul\u003e \u003cli\u003e\n\u003cstrong\u003eAntibody format:\u003c\/strong\u003e Rabbit Polyclonal Rabbit IgG\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eImmunogen \/ epitope context:\u003c\/strong\u003e E. coli-derived human VAPB recombinant protein (Position: A2-R55). Human VAPB shares 98.1% and 100% amino acid (aa) sequence identity with mouse and rat VAPB, respectively. (reported region: A2-R55).\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eMolecular weight context:\u003c\/strong\u003e reported MW: 27 kDa; calculated MW: nan\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eReactivity:\u003c\/strong\u003e Human,Mouse\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eApplications:\u003c\/strong\u003e ELISA, Flow Cytometry, IF, IHC, ICC, WB\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eAs a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.\u003c\/p\u003e \u003ch2\u003eBiological background\u003c\/h2\u003e \u003cp\u003eVAMP associated protein B and C. Vesicle-associated membrane protein-associated protein B\/C is a protein that in humans is encoded by the VAPB gene. The VAPB gene is found on the 20th human chromosome. The protein encoded by this gene is a type IV membrane protein found in plasma and intracellular vesicle membranes. The encoded protein is found as a homodimer and as a heterodimer with VAPA. This protein also can interact with VAMP1 and VAMP2 and may be involved in vesicle trafficking. Functional note: Participates in the endoplasmic reticulum unfolded protein response (UPR) by inducing ERN1\/IRE1 activity. Involved in cellular calcium homeostasis regulation. Reported localization: Endoplasmic reticulum membrane ; Present in mitochondria-associated membranes that are endoplasmic reticulum membrane regions closely apposed to the outer mitochondrial membrane. Expression\/tissue context: Ubiquitous. Isoform 1 predominates.\u003c\/p\u003e \u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e \u003cul\u003e \u003cli\u003eCancer: Researchers commonly examine how VAPB relates to this theme using model systems and orthogonal readouts.\u003c\/li\u003e\n\u003cli\u003eCancer Metabolism: Researchers commonly examine how VAPB relates to this theme using model systems and orthogonal readouts.\u003c\/li\u003e\n\u003cli\u003eLipid and Lipoprotein Metabolism: Researchers commonly examine how VAPB relates to this theme using model systems and orthogonal readouts.\u003c\/li\u003e \u003c\/ul\u003e \u003ch2\u003eCommon research applications\u003c\/h2\u003e \u003cul\u003e \u003cli\u003eWestern blotting: compare relative VAPB levels across conditions; band patterns may reflect isoforms and processing.\u003c\/li\u003e\n\u003cli\u003eIHC\/IHC-F: assess spatial distribution of VAPB across tissue regions and cell types using matched controls.\u003c\/li\u003e\n\u003cli\u003eIF\/ICC: evaluate subcellular localization and co-localization patterns; signal can depend on fixation\/permeabilization and epitope accessibility.\u003c\/li\u003e\n\u003cli\u003eFlow cytometry: quantify target-positive populations and shifts in expression; gating strategy and background staining controls are essential.\u003c\/li\u003e\n\u003cli\u003eELISA-compatible use: when applicable, interpret signal as relative abundance across sample sets with consistent handling and dilution strategy.\u003c\/li\u003e \u003c\/ul\u003e \u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e \u003cul\u003e \u003cli\u003e\n\u003cstrong\u003eSpecificity notes:\u003c\/strong\u003e No cross reactivity with other proteins.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eCross-reactivity:\u003c\/strong\u003e No cross-reactivity with other proteins.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eIsoforms and PTMs:\u003c\/strong\u003e Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eControls:\u003c\/strong\u003e Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD\/KO samples when available.\u003c\/li\u003e \u003c\/ul\u003e \u003c!-- Sources (internal): - UniProt entry for O95292: https:\/\/www.uniprot.org\/uniprotkb\/O95292\/entry - PubMed search (VAPB): https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=VAPB - NCBI Gene search (VAPB): https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=VAPB - Antibody validation concepts (NIH): https:\/\/www.nih.gov\/research-training\/rigor-reproducibility --\u003e","brand":"Boster Bio","offers":[{"title":"100 ug\/vial \/ Unconjugated","offer_id":53066869768557,"sku":"A01372","price":370.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Biotin","offer_id":53067505369453,"sku":"A01372-Biotin","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Cy3","offer_id":53067505402221,"sku":"A01372-Cy3","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro488","offer_id":53067505434989,"sku":"A01372-Fluoro488","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro550","offer_id":53067505467757,"sku":"A01372-Fluoro550","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro594","offer_id":53067505500525,"sku":"A01372-Fluoro594","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ FITC","offer_id":53067505533293,"sku":"A01372-FITC","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ HRP","offer_id":53067505566061,"sku":"A01372-HRP","price":570.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ APC","offer_id":53067505598829,"sku":"A01372-APC","price":820.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ PE","offer_id":53067505631597,"sku":"A01372-PE","price":820.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Fluoro647","offer_id":53067505664365,"sku":"A01372-Fluoro647","price":670.0,"currency_code":"USD","in_stock":true},{"title":"100 ug\/vial \/ Carrier Free","offer_id":53067505697133,"sku":"A01372-carrier-free","price":370.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/a01372-vapb-primary-antibodies-wb-testing-1_1.jpg?v=1772608379","url":"https:\/\/www.ebiohippo.com\/products\/anti-vapb-antibody-picoband-bha21001701","provider":"BioHippo","version":"1.0","type":"link"}