{"product_id":"anti-west-nile-virus-wnv-gp1-genome-polyprotein-antibody-cr4274-bha21405497","title":"Anti-West Nile virus\/WNV GP1\/Genome polyprotein Antibody (CR4274)","description":"\u003cp\u003e\u003cstrong\u003eOverview\u003c\/strong\u003e\u003cbr\u003eAntibody against West Nile virus\/WNV GP1\/Genome polyprotein (Small envelope protein M) for research detection of protein expression and localization.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eTarget biology\u003c\/strong\u003e\u003cbr\u003eWEST is a molecular target commonly interrogated by antibody-based methods to measure protein expression, localization and regulation across biological systems. Antibodies against WEST support studies ranging from baseline expression profiling to stimulus- or disease-associated changes in abundance and subcellular distribution. For pathway-level interpretation, pair WEST detection with appropriate controls and complementary readouts (e.g., genetic perturbation or orthogonal assays).\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eExpected localization\u003c\/strong\u003e\u003cbr\u003eSubcellular localization is target- and context-dependent; use cell-type appropriate controls and, where possible, genetic KO\/KD as specificity controls.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eResearch context\u003c\/strong\u003e\u003cbr\u003eThis target is frequently studied in the context of \u003cem\u003eInfectious Disease\u003c\/em\u003e research. Application suitability (e.g., ELISA, SPR, WB) is indicated in the specifications; conditions should be optimized by titration. Species reactivity information is provided in the specifications (reported: West Nile virus (strain NY-99) (WNV) (West Nile virus (strain NY-1999))).\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eRecommended experimental notes\u003c\/strong\u003e\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003eFor Western blotting, optimize lysis conditions and include reducing\/non-reducing conditions as appropriate; titrate antibody to balance sensitivity and background.\u003c\/li\u003e\n\u003cli\u003eFor ELISA, ensure capture\/detection antibody compatibility and run a standard curve; verify sample matrix effects with spike-and-recovery and dilution linearity.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eControls \u0026amp; interpretation\u003c\/strong\u003e\u003cbr\u003eWhere possible, confirm specificity using genetic perturbation (KO\/KD\/CRISPR), peptide competition (if applicable), or an orthogonal readout. Include a negative control sample expected to express low\/none of West Nile virus\/WNV GP1\/Genome polyprotein, and a positive control sample known to express West Nile virus\/WNV GP1\/Genome polyprotein.\u003c\/p\u003e\u003cp\u003e\u003cem\u003eFor research use only. Not for diagnostic procedures.\u003c\/em\u003e\u003c\/p\u003e","brand":"Biohippo Inc","offers":[{"title":"50 ug","offer_id":53036928729453,"sku":"VK554033-50UG","price":191.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53037531857261,"sku":"VK554033-100UG","price":370.0,"currency_code":"USD","in_stock":true},{"title":"1 mg","offer_id":53037531890029,"sku":"VK554033-1MG","price":1858.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/VK554033-SDS-PAGE-1.jpg?v=1771745957","url":"https:\/\/www.ebiohippo.com\/products\/anti-west-nile-virus-wnv-gp1-genome-polyprotein-antibody-cr4274-bha21405497","provider":"BioHippo","version":"1.0","type":"link"}