{"product_id":"anxa2-antibody-annexin-a2-bha17135823","title":"ANXA2 Antibody \/ Annexin A2","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eANXA2 Antibody \/ Annexin A2 is a anti-ANXA2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e ANXA2\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eANXA2 antibody detects Annexin A2, a calcium-dependent phospholipid-binding protein involved in membrane organization, cytoskeletal interactions, and fibrinolysis. Encoded by the ANXA2 gene on chromosome 15q26.1, this multifunctional protein participates in membrane trafficking, endocytosis, cell-cell adhesion, and extracellular matrix remodeling. Annexin A2 also acts as a cell surface receptor for tissue plasminogen activator (tPA) and plasminogen, facilitating localized fibrin degradation and vascular repair.\u003cbr\u003e\u003cbr\u003eStructurally, Annexin A2 is a 339-amino-acid protein of approximately 39 kilodaltons containing four annexin repeats that mediate calcium-dependent phospholipid binding and a unique N-terminal domain that interacts with S100A10 (p11) to form a heterotetrameric complex. This complex localizes to the plasma membrane, cytoskeleton, and endosomal compartments, where it modulates vesicle fusion, cytoskeletal anchoring, and membrane curvature. Annexin A2 is expressed in endothelial, epithelial, and tumor cells, as well as macrophages and neurons.\u003cbr\u003e\u003cbr\u003eThe ANXA2 antibody is widely used in cell biology, vascular biology, and cancer research to study membrane organization, signal transduction, and fibrinolytic regulation. Western blot analysis detects a 39 kilodalton band corresponding to Annexin A2, while immunofluorescence shows cytoplasmic, membrane, and perinuclear localization. This antibody provides a powerful reagent for investigating calcium-dependent membrane remodeling and extracellular proteolytic activity.\u003cbr\u003e\u003cbr\u003eFunctionally, Annexin A2 regulates actin cytoskeleton rearrangement and vesicle trafficking, influencing endocytosis, exocytosis, and cell motility. At the cell surface, it promotes plasmin generation, contributing to fibrin degradation, angiogenesis, and tumor invasion. Dysregulation of ANXA2 expression is associated with cancer progression, thrombosis, and inflammatory disease. The ANXA2 antibody supports detailed studies of its role in cellular dynamics, extracellular matrix remodeling, and vascular homeostasis.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=ANXA2 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=ANXA2 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=ANXA2 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/ANXA2 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=ANXA2+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047306256749,"sku":"FY12921","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_5788045b-0762-44a9-bae0-cdcb7b8899e7.jpg?v=1782237056","url":"https:\/\/www.ebiohippo.com\/products\/anxa2-antibody-annexin-a2-bha17135823","provider":"BioHippo","version":"1.0","type":"link"}