{"product_id":"ap1-reporter-lentivirus-bhv19400017","title":"AP1 Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eActivator protein 1 (AP-1) is a dimeric transcription factor formed by members of the JUN, FOS, ATF, and MAF protein families. AP-1 binds TPA- and cAMP-responsive DNA elements to regulate genes controlling cell proliferation, differentiation, survival, and stress responses. AP-1 activity is a major downstream output of the c-Jun N-terminal kinase (JNK) MAP kinase cascade: JNK phosphorylates c-Jun and related factors to enhance their transcriptional activity. The JNK-AP-1 axis is activated by growth factors, cytokines, and cellular stress, and its dysregulation contributes to inflammation, oncogenesis, and apoptosis, making AP-1-driven reporters valuable tools for studying stress and mitogenic signaling.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe AP1 Reporter Lentivirus is a transcription factor reporter system that provides a sensitive fluorescent or luminescent readout of AP-1 transcriptional activity in human or mouse cells, reflecting JNK signaling pathway activation. Reporter expression is controlled by AP-1-responsive elements, so signal intensity tracks endogenous AP-1 activity. Stable lentiviral integration enables generation of polyclonal reporter cell lines for analysis by fluorescence microscopy, flow cytometry, or luminometry. The system is used to study JNK\/AP-1 signaling in stress responses, proliferation, inflammation, and apoptosis, and to screen pathway modulators. Particles are purified by PEG precipitation and sucrose gradient centrifugation, supporting efficient transduction of difficult-to-transfect cells, including primary and cryopreserved cultures.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a Firefly Luc, GFP, Luc, Renilla Luc, RFP reporter gene under the control of tandem consensus response elements specific for the JNK signaling pathway transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Puromycin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53251617128813,"sku":"LTV-0019-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53310896341357,"sku":"LTV-0019-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Puromycin \/ 2x10^6","offer_id":53310896374125,"sku":"LTV-0019-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Blasticidin \/ 2x10^6","offer_id":53310896406893,"sku":"LTV-0019-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53310896439661,"sku":"LTV-0019-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53310896472429,"sku":"LTV-0019-6S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Renilla Luc \/ Blasticidin \/ 2x10^6","offer_id":53310896505197,"sku":"LTV-0019-4SIC","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/TF-Reporter-Vector-circular_87b181c9-7175-475e-869d-e05b5a9fab38.jpg?v=1776934280","url":"https:\/\/www.ebiohippo.com\/products\/ap1-reporter-lentivirus-bhv19400017","provider":"BioHippo","version":"1.0","type":"link"}