{"product_id":"ap2-reporter-lentivirus-bhv19400250","title":"AP2 Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe AP-2 family of transcription factors, encoded by genes including TFAP2A, TFAP2B, and TFAP2C, are key regulators of development. They are strongly expressed in neural crest cells and their derivatives, where they control facial and craniofacial development, limb patterning, and organogenesis. AP-2 factors bind GC-rich DNA elements and influence cell proliferation, differentiation, and apoptosis across multiple tissues. Loss or dysregulation of AP-2 activity is associated with developmental disorders, while altered AP-2 function also contributes to tumorigenesis in several cancers. Because of these roles, AP-2-dependent transcription is an informative readout in developmental biology and cell signaling research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe AP2 Reporter Lentivirus is a transcription-factor reporter system that detects transcriptional activity mediated by AP-2 (TFAP2) factors in human and mouse cells. The construct uses tandem repeats of AP-2 DNA-binding elements derived from the human metallothionein IIa promoter coupled to a minimal promoter to drive a fluorescent or luminescent reporter, with options including GFP, RFP, mCherry, BFP2, and luciferase configurations. A constitutively expressed selection marker supports stable polyclonal cell line generation.\u003c\/p\u003e\n\u003cp\u003eReadout is by microscopy, flow cytometry, or luminometry. Applications include studying neural crest development, AP-2 regulation of differentiation, and AP-2 dysregulation in disease. Particles are purified by PEG precipitation and sucrose gradient centrifugation for transduction of primary and thawed cells.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a BFP2, d2GFP, EGFP, Firefly Luc, Gaussia Luc, GFP, GFP + Firefly Luc, mCherry, Renilla Luc, RFP, RFP + Firefly Luc reporter gene under the control of tandem consensus response elements specific for the AP-2 Pathway transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Hygromycin, Puromycin, Zeocin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"AP-2-TAG-Puro \/ GFP \/ 5x10^6","offer_id":53251615785325,"sku":"LTV-0143-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Negative Control (NC-TAG-Puro) \/ GFP \/ 5x10^6","offer_id":53362091884909,"sku":"LTV-0143-1N","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Positive Control (PC-TAG-Puro) \/ GFP \/ 5x10^6","offer_id":53362091917677,"sku":"LTV-0143-1P","price":595.0,"currency_code":"USD","in_stock":true},{"title":"AP-2-TAL-Puro \/ Firefly Luc \/ 2x10^6","offer_id":53362091950445,"sku":"LTV-0143-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Negative Control (NC-TAL-Puro) \/ Firefly Luc \/ 2x10^6","offer_id":53362091983213,"sku":"LTV-0143-3N","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Positive Control (PC-TAL-Puro) \/ Firefly Luc \/ 2x10^6","offer_id":53362092015981,"sku":"LTV-0143-3P","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Internal Control (RLuc-BSD) \/ Renilla Luc \/ 2x10^6","offer_id":53362092048749,"sku":"LTV-0143-3R","price":595.0,"currency_code":"USD","in_stock":true},{"title":"AP-2-TAR-BSD \/ RFP \/ 5x10^6","offer_id":53362092081517,"sku":"LTV-0143-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"AP-2-TAL-BSD \/ Firefly Luc \/ 2x10^6","offer_id":53362092114285,"sku":"LTV-0143-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"AP-2-TAG-BSD \/ GFP \/ 5x10^6","offer_id":53362092147053,"sku":"LTV-0143-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"AP-2-TAR-Puro \/ RFP \/ 5x10^6","offer_id":53362092179821,"sku":"LTV-0143-6S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/AP2-TAG-PURO-THUMB.jpg?v=1776934133","url":"https:\/\/www.ebiohippo.com\/products\/ap2-reporter-lentivirus-bhv19400250","provider":"BioHippo","version":"1.0","type":"link"}