{"product_id":"ap2a1-antibody-bha10557042","title":"AP2A1 Antibody","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eThis is a polyclonal anti-AP2A1 antibody raised in Rabbit, with confirmed utility in ELISA, WB, IHC. It is designed to detect AP2A1 protein in Human, Mouse and supports researchers working in signal transduction contexts.\u003c\/p\u003e\n\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunogen:\u003c\/strong\u003e Synthetic peptide of Human AP2A1 — determines the epitope region; confirm compatibility with sample preparation and expected post-translational modifications.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHost species (Rabbit):\u003c\/strong\u003e Requires anti-rabbit-IgG secondary reagents for indirect detection.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePolyclonal format:\u003c\/strong\u003e Recognizes multiple epitopes for robust signal; inherent lot-to-lot variability requires appropriate controls.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eIsotype (IgG):\u003c\/strong\u003e Matched secondary antibodies and isotype controls required. Compatible with standard Protein A\/G purification workflows.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurification (Antigen affinity purification):\u003c\/strong\u003e Enriches for specific immunoglobulin classes; reduces non-specific background vs. crude antisera.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eAP2A1\u003c\/strong\u003e (also referred to as 100 kDa coated vesicle protein A, Adapter-related protein complex 2 alpha-1 subunit, adapter-related protein complex 2 subunit alpha-1, Adaptin alpha A, Adaptin alpha, Adaptor protein complex AP 2 alpha 1 subunit, adaptor protein complex AP 2 subunit alpha 1, Adaptor protein complex AP-2 subunit alpha-1, Adaptor protein complex AP2 alpha 1 subunit, adaptor protein complex AP2 subunit alpha 1, Adaptor related protein complex 2 alpha 1 subunit, Adaptor related protein complex AP 2 alpha 1 subunit, Adaptor related protein complex AP2 alpha 1 subunit, ADTAA, Alpha A adaptin, Alpha adaptin A, Alpha-adaptin A, alpha1 adaptin, Alpha1-adaptin, AP 2 alpha, AP 2 complex subunit alpha 1, AP-2 complex subunit alpha-1, AP2 complex subunit alpha 1, AP2-ALPHA, Ap2a1, AP2A1_HUMAN, CLAPA 1, CLAPA1, Clathrin adaptor complex AP2. alpha subunit, Clathrin assembly protein complex 2 alpha A large chain, Clathrin assembly protein complex 2 alpha-A large chain, Clathrin associated \/ assembly \/ adaptor protein large alpha 1, clathrin associated assembly adaptor protein large alpha 1, clathrin associated\/assembly\/adaptor protein, large, alpha 1, Plasma membrane adaptor HA 2\/AP 2 adaptin alpha A subunit, Plasma membrane adaptor HA2\/AP2 adaptin alpha A subunit) is a protein target studied in Human systems. Expression, subcellular localization, and post-translational modifications vary across cell types and disease states — factors critical to antibody-based detection design. Consult UniProt, NCBI Gene, and primary literature for current annotation of AP2A1 biology in signal transduction.\u003c\/p\u003e\n\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e Quantification of soluble target in biological fluids or culture supernatants. Ensure samples are within the linear detection range.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWB:\u003c\/strong\u003e Confirms target molecular weight and distinguishes isoforms under denaturing conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eIHC:\u003c\/strong\u003e Visualization of target in FFPE or frozen tissue sections. Optimize antigen retrieval and secondary detection for each tissue type.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eIsotype controls:\u003c\/strong\u003e Use an isotype-matched (IgG from Rabbit) control at equivalent concentration to assess non-specific background.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCross-reactivity:\u003c\/strong\u003e Polyclonal preparations may cross-react with related proteins. Orthogonal validation (siRNA, KO lysate) is recommended.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMatrix effects:\u003c\/strong\u003e Sample matrix can affect performance; pilot dilution linearity and spike-recovery experiments are advised for quantitative studies.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSpecies reactivity:\u003c\/strong\u003e Confirmed for Human, Mouse. Extrapolation to untested species requires empirical validation.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003cp\u003e\u003cem\u003eThis product is a polyclonal antibody purified from rabbit antiserum.\u003c\/em\u003e\u003c\/p\u003e\n\n\u003c!-- Sources:\n- UniProt: https:\/\/www.uniprot.org\n- NCBI Gene: https:\/\/www.ncbi.nlm.nih.gov\/gene\n- Cusabio: https:\/\/www.cusabio.com\n- Human Protein Atlas: https:\/\/www.proteinatlas.org\n- PubMed: https:\/\/pubmed.ncbi.nlm.nih.gov\n--\u003e","brand":"CUSABIO TECHNOLOGY LLC","offers":[{"title":"50 uL","offer_id":53247523651949,"sku":"CSB-PA086984-50UL","price":170.98,"currency_code":"USD","in_stock":true},{"title":"100 uL","offer_id":53247613469037,"sku":"CSB-PA086984-100UL","price":307.97,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/CSB-PA086984-P.jpg?v=1776788239","url":"https:\/\/www.ebiohippo.com\/products\/ap2a1-antibody-bha10557042","provider":"BioHippo","version":"1.0","type":"link"}