{"product_id":"ap2m1-antibody-ap-2-complex-subunit-mu-bha17136347","title":"AP2M1 Antibody \/ AP-2 complex subunit mu","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eAP2M1 Antibody \/ AP-2 complex subunit mu is a anti-AP2M1 Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB), IHC-P, Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm, cell membrane.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e AP2M1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Antigen affinity purified\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC-P, IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eAP2M1 (Adaptor-related protein complex 2 subunit mu 1, AP-2 complex subunit mu) is a critical component of the adaptor protein 2 (AP-2) complex, which is essential for clathrin-mediated endocytosis. This process is responsible for the internalization of receptors, nutrients, and other molecules from the plasma membrane into endocytic vesicles. AP2M1 acts as the medium (mu) subunit of the complex and is directly involved in cargo recognition by binding to specific endocytic motifs on membrane proteins. A AP2M1 antibody is frequently used to study endocytosis, receptor trafficking, and membrane dynamics.    \u003cbr\u003e\u003cbr\u003e  The AP-2 complex consists of two large subunits (alpha and beta), one medium subunit (mu, encoded by AP2M1), and one small subunit (sigma). Together, they coordinate clathrin recruitment and vesicle formation. AP2M1 is particularly important because of its ability to recognize tyrosine-based sorting signals on transmembrane receptors, ensuring selective cargo uptake. Employing a AP2M1 antibody allows researchers to examine receptor internalization, vesicle trafficking, and signal transduction.    \u003cbr\u003e\u003cbr\u003e  Alterations in AP2M1 function have been implicated in neurological disorders and developmental abnormalities, as proper endocytic trafficking is vital for synaptic vesicle recycling and neuronal signaling. Dysregulation of AP2M1-mediated pathways may also contribute to cancer progression, viral entry, and other disease processes. Using a AP2M1 antibody provides valuable insights into these mechanisms and helps researchers explore therapeutic strategies targeting endocytic pathways.    \u003cbr\u003e\u003cbr\u003e\n\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=AP2M1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=AP2M1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=AP2M1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/AP2M1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=AP2M1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"0.5mg\/ml if reconstituted with 0.2ml sterile DI water \/ 100 ug","offer_id":53047326146925,"sku":"RQ8929","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_ab09ef70-138e-450e-8f6b-c0de563df487.jpg?v=1772019448","url":"https:\/\/www.ebiohippo.com\/products\/ap2m1-antibody-ap-2-complex-subunit-mu-bha17136347","provider":"BioHippo","version":"1.0","type":"link"}