{"product_id":"apoa1-antibody-apolipoprotein-a-bha17135505","title":"APOA1 Antibody \/ Apolipoprotein A","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eAPOA1 Antibody \/ Apolipoprotein A is a anti-APOA1 Mouse antibody Monoclonal (mouse origin) clone 17G5 supplied in Lyophilized format. Recommended for workflows such as ELISA (Cap), Western blot (WB) with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e APOA1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Mouse, Monoclonal (mouse origin), clone 17G5, isotype Mouse IgG1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e ELISA (Cap), WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eAPOA1 antibody detects Apolipoprotein A-I, the major protein component of high-density lipoprotein (HDL) that mediates cholesterol transport and lipid metabolism. APOA1 facilitates reverse cholesterol transport by binding cellular cholesterol and phospholipids, delivering them to the liver for excretion. The APOA1 antibody is widely used in cardiovascular, metabolic, and lipid biology research to study HDL function, atherosclerosis prevention, and lipid-protein interactions.\u003cbr\u003e\u003cbr\u003eAPOA1 is encoded by the APOA1 gene located on human chromosome 11q23.3. The protein is approximately 243 amino acids long and synthesized mainly in the liver and small intestine. APOA1 circulates in plasma as part of HDL particles, where it activates lecithin-cholesterol acyltransferase (LCAT), an enzyme that esterifies free cholesterol, promoting HDL maturation. It plays a central role in maintaining lipid balance and preventing lipid accumulation in arterial walls.\u003cbr\u003e\u003cbr\u003eThe APOA1 antibody detects a 28 kilodalton band by western blot and exhibits strong cytoplasmic and extracellular staining in hepatic and intestinal tissues. APOA1 interacts with ATP-binding cassette transporters ABCA1 and ABCG1 to facilitate cholesterol efflux from peripheral cells. It also exhibits antioxidant and anti-inflammatory functions by neutralizing oxidized lipids and modulating cytokine signaling, contributing to cardiovascular protection.\u003cbr\u003e\u003cbr\u003eMutations or decreased expression of APOA1 lead to hypoalphalipoproteinemia and increased risk of coronary artery disease. Conversely, elevated APOA1 levels correlate with improved lipid clearance and reduced atherosclerotic plaque formation. In addition to lipid transport, APOA1 influences glucose metabolism, immune modulation, and amyloid aggregation prevention.\u003cbr\u003e\u003cbr\u003eAs a multifunctional lipid carrier and protective factor in cardiovascular health, APOA1 serves as both a biomarker and therapeutic target for metabolic disorders.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=APOA1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=APOA1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=APOA1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/APOA1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=APOA1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047294493037,"sku":"FY12602","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_d8576271-fd8f-4247-8262-646781a9af56.jpg?v=1782237025","url":"https:\/\/www.ebiohippo.com\/products\/apoa1-antibody-apolipoprotein-a-bha17135505","provider":"BioHippo","version":"1.0","type":"link"}