{"product_id":"aqp2-antibody-aquaporin-2-bha17135321","title":"Aqp2 Antibody \/ Aquaporin 2","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eAqp2 Antibody \/ Aquaporin 2 is a anti-AQP2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e AQP2\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eThe AQP2 antibody targets Aquaporin-2, a water channel protein encoded by the AQP2 gene. Aquaporin-2 is an essential component of the kidney's water reabsorption system, located in the apical membrane of collecting duct principal cells. It mediates vasopressin-regulated water transport, thereby controlling urine concentration and maintaining body fluid balance. The AQP2 antibody enables researchers to study renal physiology, osmoregulation, and disorders of water homeostasis such as diabetes insipidus.\u003cbr\u003e\u003cbr\u003eAquaporin-2 belongs to the aquaporin family of integral membrane proteins that selectively transport water across biological membranes. Under the influence of the antidiuretic hormone vasopressin, AQP2-containing vesicles are trafficked to and fused with the apical plasma membrane, increasing water permeability. The AQP2 antibody provides a reliable means to visualize this regulated translocation process, making it a key tool for examining vasopressin signaling and collecting duct responsiveness.\u003cbr\u003e\u003cbr\u003eMutations in the AQP2 gene lead to nephrogenic diabetes insipidus (NDI), characterized by excessive urine production and an inability to concentrate urine despite normal vasopressin levels. The AQP2 antibody supports research into this condition by enabling detection of defective or mislocalized Aquaporin-2 in renal tissue. Experimental models show that AQP2 deficiency disrupts water retention and contributes to dehydration, underscoring its clinical relevance in renal water management.\u003cbr\u003e\u003cbr\u003eBeyond kidney physiology, Aquaporin-2 has been detected in reproductive tissues and the central nervous system, suggesting broader roles in fluid transport and homeostasis. The AQP2 antibody supports such investigations, allowing comparative expression analysis across tissues and experimental conditions. Regulation of AQP2 expression and trafficking involves multiple pathways including cyclic AMP, protein kinase A, and ubiquitination-dependent endocytosis.\u003cbr\u003e\u003cbr\u003eThe AQP2 antibody performs effectively in western blotting, immunohistochemistry, and immunofluorescence, producing distinct apical membrane staining in kidney tissue.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=AQP2 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=AQP2 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=AQP2 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/AQP2 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=AQP2+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047286694253,"sku":"FY12418","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_04a290a3-0bc6-47c8-ac9b-ec704bc85e8c.jpg?v=1782236992","url":"https:\/\/www.ebiohippo.com\/products\/aqp2-antibody-aquaporin-2-bha17135321","provider":"BioHippo","version":"1.0","type":"link"}