{"product_id":"are-luc-cmvh-stably-expressing-raw264-7-cell-line-bhc10923268","title":"ARE‐luc‐CMVH Stably Expressing RAW264.7 Cell Line","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eThe RAW-ARE-luc-CMVH Stable RAW264.7 Cell Line is specifically designed for the study of the antioxidant response element (ARE) pathway, a crucial mechanism in cellular defense against oxidative stress. This cell line, based on the RAW264.7 murine macrophage lineage, stably incorporates a luciferase reporter gene under the control of an ARE-responsive promoter, enhanced by a cytomegalovirus (CMV) enhancer.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eModel identity:\u003c\/strong\u003e ARE‐luc‐CMVH Stably Expressing RAW264.7 Cell Line is supplied as a tumor cell line derived from Mouse ascites.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Adherent, dendritic-like\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth conditions:\u003c\/strong\u003e PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. MEM α, No Nucleosides Medium (TM512) + 10% FBS(Regular*) + 2 mM L-Glutamine (G275) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. *Do not heat-inactivate\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eEngineering \/ immortalization:\u003c\/strong\u003e Luciferase reporter expression.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct format:\u003c\/strong\u003e Frozen, BSL-2\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis cell-based model is generally used in neurobiology, differentiation, and cell signaling studies. Donor\/background information is available for contextual interpretation.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eThis setup enables precise, real-time tracking of ARE activation in response to oxidative stressors and toxicological compounds. It is particularly useful in pharmacological research, allowing for the screening and evaluation of drugs that modulate oxidative stress responses, potentially offering therapeutic strategies for diseases characterized by oxidative damage, such as neurodegenerative disorders and cancer. The cell line ensures consistent performance with its stable reporter gene integration, facilitating reliable and reproducible results in studies of oxidative stress and cellular defense mechanisms.Complete list of cell lines from this panel:Cat. T6529 - NFAT‐luc‐CMVH Stably Expressing RAW264.7 Cell LineCat. T6530 - AP‐1‐luc‐CMVH Stably Expressing RAW264.7 Cell LineCat. T6531 - ARE‐luc‐CMVH Stably Expressing RAW264.7 Cell Line Donor\/background information provided for this product: Male, Balb\/c, Adult, Abelson murine leukemia virus-induced tumor.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCell-line models continue to be used for tumor phenotype comparison, pathway perturbation studies, and assay development in controlled in vitro systems.\u003c\/li\u003e\n\u003cli\u003eEngineered and subtype-defined tumor lines are often used to compare growth behavior, reporter output, and response patterns across matched experimental conditions.\u003c\/li\u003e\n\u003cli\u003eWhen metastatic or lineage features are described, investigators commonly interpret results alongside morphology, passage history, and culture environment.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eNeurobiology-focused studies of growth state, differentiation-associated morphology, and cell signaling changes in culture.\u003c\/li\u003e\n\u003cli\u003eCell-based assays that compare experimental perturbations across defined media and substrate conditions.\u003c\/li\u003e\n\u003cli\u003ePhenotype tracking using morphology, marker expression, or reporter output where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eChanges in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMorphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.\u003c\/li\u003e\n\u003cli\u003eMatched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCulture and product details\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. MEM α, No Nucleosides Medium (TM512) + 10% FBS(Regular*) + 2 mM L-Glutamine (G275) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. *Do not heat-inactivate\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWarranty:\u003c\/strong\u003e abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and\/or catalog description. Such thirty (30) day period is referred to herein as the \"Warranty Period”.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53180552348013,"sku":"T6531","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/1FNz4w0ru3IIer50dRLKEVs7VdZtkbzcDYRBDye7.png?v=1774957888","url":"https:\/\/www.ebiohippo.com\/products\/are-luc-cmvh-stably-expressing-raw264-7-cell-line-bhc10923268","provider":"BioHippo","version":"1.0","type":"link"}