{"product_id":"are-reporter-lentivirus-bhv19400016","title":"ARE Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe antioxidant response element (ARE) is a cis-regulatory DNA sequence found in the promoters of genes encoding detoxification and antioxidant enzymes. It is the principal binding site for NFE2L2 (NRF2) and the related factor NFE2L1 (NRF1), basic leucine zipper transcription factors that orchestrate the cellular defense against oxidative and electrophilic stress. Under basal conditions NRF2 is held in the cytoplasm by KEAP1 and targeted for degradation; oxidative stress stabilizes NRF2, allowing it to enter the nucleus, dimerize with small MAF proteins, and activate ARE-driven transcription. This cytoprotective program is central to redox homeostasis, and its dysregulation contributes to inflammation, neurodegeneration, and cancer.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe ARE Reporter Lentivirus is a lentiviral reporter system for monitoring NRF1\/NRF2 antioxidant signaling in human or mouse cells. An antioxidant response element placed upstream of a fluorescent or luminescent reporter (GFP, RFP, firefly luciferase, with Renilla luciferase available) provides a sensitive readout of NRF1\/2 transcriptional activity, enabling detection of antioxidant pathway activation in transduced cells. Optional antibiotic selection markers (puromycin or blasticidin) support generation of stable reporter cell lines. The product is well suited to studying oxidative stress responses, screening NRF2 activators or inhibitors, and characterizing redox signaling. Supplied as lentiviral particles purified by PEG precipitation and sucrose gradient centrifugation, it efficiently transduces difficult-to-transfect cells, including primary and thawed cells.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a Firefly Luc, GFP, Luc, Renilla Luc, RFP reporter gene under the control of tandem consensus response elements specific for the NRF1\/2 antioxidant pathway transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Puromycin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53251622994285,"sku":"LTV-0018-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53310900142445,"sku":"LTV-0018-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Puromycin \/ 2x10^6","offer_id":53310900175213,"sku":"LTV-0018-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Blasticidin \/ 2x10^6","offer_id":53310900207981,"sku":"LTV-0018-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53310900240749,"sku":"LTV-0018-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53310900273517,"sku":"LTV-0018-6S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Renilla Luc \/ Blasticidin \/ 2x10^6","offer_id":53310900306285,"sku":"LTV-0018-4SIC","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/LTV-0018-ARE-Reporter-Fig.2-NRF-6pa-orig.jpg?v=1776934760","url":"https:\/\/www.ebiohippo.com\/products\/are-reporter-lentivirus-bhv19400016","provider":"BioHippo","version":"1.0","type":"link"}