{"product_id":"arhgef2-antibody-rho-guanine-nucleotide-exchange-factor-2-gef-h1-bha17135964","title":"ARHGEF2 Antibody \/ Rho guanine nucleotide exchange factor 2 \/ GEF-H1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eARHGEF2 Antibody \/ Rho guanine nucleotide exchange factor 2 \/ GEF-H1 is a anti-ARHGEF2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e ARHGEF2\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eARHGEF2 antibody detects Rho guanine nucleotide exchange factor 2, also known as GEF-H1, a microtubule-associated protein that activates Rho family GTPases to regulate cytoskeletal organization and cell polarity. The UniProt recommended name is Rho guanine nucleotide exchange factor 2 (ARHGEF2). This signaling factor converts Rho GTPases from inactive GDP-bound to active GTP-bound states, controlling cell shape, motility, and junctional dynamics.\u003cbr\u003e\u003cbr\u003eFunctionally, ARHGEF2 antibody identifies a 986-amino-acid cytoplasmic protein containing Dbl homology (DH) and pleckstrin homology (PH) domains responsible for its guanine nucleotide exchange activity. ARHGEF2 activates RHOA and RHOC, stimulating actin stress fiber formation, contractility, and focal adhesion turnover. When bound to microtubules, ARHGEF2 remains inactive; upon microtubule depolymerization, it is released into the cytoplasm, where it activates RHOA signaling.\u003cbr\u003e\u003cbr\u003eThe ARHGEF2 gene is located on chromosome 1q22 and encodes a protein expressed in epithelial cells, neurons, and endothelial tissues. ARHGEF2 plays a key role in linking microtubule dynamics to actin cytoskeletal remodeling, influencing cell junction integrity and migration. In epithelial cells, it regulates tight junction assembly and polarity through interactions with PAR6 and AMPK. In neurons, ARHGEF2 contributes to axon guidance and dendritic spine morphology.\u003cbr\u003e\u003cbr\u003ePathologically, dysregulation of ARHGEF2 activity has been associated with cancer progression, inflammation, and neurodevelopmental disorders. Overactivation of RHOA signaling by ARHGEF2 promotes tumor invasion, while loss of its regulation can impair barrier function in inflammatory bowel disease. ARHGEF2 also mediates cytokine-induced cytoskeletal remodeling during immune responses, linking microtubule organization to inflammatory signaling pathways.\u003cbr\u003e\u003cbr\u003eARHGEF2 antibody is widely used in cytoskeletal, cell signaling, and cancer biology research. It is suitable for immunoblotting, immunofluorescence, and immunoprecipitation to detect ARHGEF2 expression and RHOA activation states. This antibody supports studies of cell polarity, microtubule-actin crosstalk, and epithelial integrity. In biomedical research, ARHGEF2 serves as a molecular bridge between cytoskeletal regulation and signal transduction.\u003cbr\u003e\u003cbr\u003eStructurally, ARHGEF2 contains modular domains for RHOA interaction, microtubule binding, and regulation by phosphorylation. Its activity is modulated by kinases including MARK2 and Aurora A, which control its release from microtubules.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=ARHGEF2 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=ARHGEF2 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=ARHGEF2 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/ARHGEF2 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=ARHGEF2+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047310877037,"sku":"FY13062","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_c5b77af7-3229-4756-8db8-6ea58c4fe161.jpg?v=1782237082","url":"https:\/\/www.ebiohippo.com\/products\/arhgef2-antibody-rho-guanine-nucleotide-exchange-factor-2-gef-h1-bha17135964","provider":"BioHippo","version":"1.0","type":"link"}