{"product_id":"arhgef6-antibody-rho-guanine-nucleotide-exchange-factor-6-bha17136074","title":"ARHGEF6 Antibody \/ Rho guanine nucleotide exchange factor 6","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eARHGEF6 Antibody \/ Rho guanine nucleotide exchange factor 6 is a anti-ARHGEF6 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e ARHGEF6\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eARHGEF6 antibody detects Rho guanine nucleotide exchange factor 6, a signaling protein that activates Rho family GTPases to control cytoskeletal organization, cell migration, and synaptic plasticity. The UniProt recommended name is Rho guanine nucleotide exchange factor 6 (ARHGEF6). This protein, also known as alpha-PIX or Cool-2, acts as an exchange factor for RAC1 and CDC42, converting them from GDP-bound inactive forms to GTP-bound active states.\u003cbr\u003e\u003cbr\u003eFunctionally, ARHGEF6 antibody identifies a 776-amino-acid scaffold protein containing SH3, DH, and PH domains that mediate interaction with PAK kinases and other signaling adaptors. ARHGEF6 promotes actin cytoskeleton rearrangements, lamellipodia formation, and cell adhesion dynamics through activation of Rho GTPase signaling cascades.\u003cbr\u003e\u003cbr\u003eThe ARHGEF6 gene is located on chromosome Xq26.3 and is highly expressed in the brain, immune cells, and epithelial tissues. It participates in synaptic development, immune cell migration, and epithelial junction organization by coupling surface receptors to cytoskeletal effectors.\u003cbr\u003e\u003cbr\u003ePathologically, mutations in ARHGEF6 cause X-linked intellectual disability due to impaired synaptic signaling and dendritic spine morphology. Aberrant ARHGEF6 expression is also associated with immune dysfunction and metastatic cancer cell migration. Research using ARHGEF6 antibody supports studies in cell signaling, cytoskeletal regulation, and neurological disorders.\u003cbr\u003e\u003cbr\u003eARHGEF6 antibody is validated for western blotting, immunofluorescence, and immunohistochemistry to detect guanine nucleotide exchange factors. \u003cbr\u003e\u003cbr\u003eStructurally, Rho guanine nucleotide exchange factor 6 forms multiprotein complexes with PAK kinases and GIT proteins, linking receptor activation to localized actin assembly. This antibody enables exploration of ARHGEF6's molecular function in Rho GTPase activation and cellular morphogenesis.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=ARHGEF6 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=ARHGEF6 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=ARHGEF6 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/ARHGEF6 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=ARHGEF6+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047314481517,"sku":"FY13172","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_0166b996-98bd-4357-bf88-c55ae2a5ca30.jpg?v=1782237113","url":"https:\/\/www.ebiohippo.com\/products\/arhgef6-antibody-rho-guanine-nucleotide-exchange-factor-6-bha17136074","provider":"BioHippo","version":"1.0","type":"link"}