{"product_id":"arl3-antibody-adp-ribosylation-factor-like-protein-3-bha17135332","title":"ARL3 Antibody \/ ADP-ribosylation factor-like protein 3","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eARL3 Antibody \/ ADP-ribosylation factor-like protein 3 is a anti-ARL3 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear, cytoplasmic.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e ARL3\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC, IF, FACS\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eThe ARL3 antibody targets ADP-ribosylation factor-like protein 3, a small GTP-binding protein encoded by the ARL3 gene. ADP-ribosylation factor-like protein 3 is a member of the Arf family of GTPases that regulates trafficking of lipid-modified proteins to primary cilia and photoreceptor outer segments. Through its role in ciliary cargo transport, ARL3 ensures the correct localization of proteins essential for sensory signaling and phototransduction. The ARL3 antibody provides a reliable reagent for studying ciliary trafficking, GTPase signaling, and inherited retinal disorders.\u003cbr\u003e\u003cbr\u003eADP-ribosylation factor-like protein 3 cycles between GTP- and GDP-bound states, regulating the release of lipidated cargo from carrier proteins such as PDE6D and UNC119. The ARL3 antibody supports studies that monitor these molecular interactions and visualize ARL3 localization within cilia, photoreceptor cells, and centrosomes. Its activation is mediated by the guanine nucleotide exchange factor ARL13B, while GTP hydrolysis is stimulated by the GTPase-activating protein RP2. Together, these interactions ensure precise spatiotemporal control of cargo delivery.\u003cbr\u003e\u003cbr\u003eMutations in the ARL3 gene cause Joubert syndrome and cone-rod dystrophy, disorders associated with ciliary dysfunction and photoreceptor degeneration. The ARL3 antibody is critical for studying these ciliopathies, enabling detection of expression changes and subcellular mislocalization of mutant proteins. Disruption of ARL3-mediated trafficking leads to accumulation of mislocalized proteins and progressive loss of photoreceptor function.\u003cbr\u003e\u003cbr\u003eBeyond the retina, ADP-ribosylation factor-like protein 3 regulates microtubule organization and vesicle transport in other ciliated and non-ciliated cells. The ARL3 antibody supports investigations into these broader cellular functions, including roles in signal transduction and organelle positioning. ARL3 also contributes to lipid modification cycles that control the targeting of myristoylated and prenylated proteins.\u003cbr\u003e\u003cbr\u003eThe ARL3 antibody performs effectively in western blotting, immunofluorescence, and immunohistochemistry, displaying distinct ciliary and pericentrosomal staining consistent with its trafficking role.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=ARL3 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=ARL3 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=ARL3 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/ARL3 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=ARL3+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047287578989,"sku":"FY12429","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_c684fad7-a47a-48be-bec9-82e81c1b9455.jpg?v=1782237005","url":"https:\/\/www.ebiohippo.com\/products\/arl3-antibody-adp-ribosylation-factor-like-protein-3-bha17135332","provider":"BioHippo","version":"1.0","type":"link"}