{"product_id":"arpin-antibody-actin-related-protein-complex-inhibitor-bha17136140","title":"ARPIN Antibody \/ Actin-related protein complex inhibitor","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eARPIN Antibody \/ Actin-related protein complex inhibitor is a anti-ARPIN Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS) with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e ARPIN\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eARPIN antibody detects Actin-related protein complex inhibitor (Arpin), a negative regulator of actin filament branching that modulates cell motility and migration. The UniProt recommended name is Actin-related protein complex inhibitor (ARPIN). This cytoplasmic protein inhibits the Arp2\/3 complex, controlling actin polymerization dynamics essential for lamellipodia formation, directional movement, and membrane protrusion regulation.\u003cbr\u003e\u003cbr\u003eFunctionally, ARPIN antibody identifies a 220-amino-acid protein that binds directly to the Arp2\/3 complex through its C-terminal acidic motif, competing with activating factors such as WASP and WAVE. By inhibiting Arp2\/3-dependent actin nucleation, ARPIN reduces protrusive force at the cell leading edge, promoting turning behavior and directional persistence. The protein is involved in establishing cell polarity, controlling migration speed, and maintaining cytoskeletal balance between extension and contraction.\u003cbr\u003e\u003cbr\u003eThe ARPIN gene (also known as C15orf38) is located on chromosome 15q24.1 and is expressed in multiple tissues, particularly in epithelial cells, fibroblasts, and neurons. Its activity is tightly regulated by upstream signaling through Rac1 and PI3K pathways that coordinate motility and cell shape remodeling.\u003cbr\u003e\u003cbr\u003ePathologically, altered ARPIN expression affects wound healing, cancer cell migration, and metastasis. Downregulation enhances cell motility and invasion by releasing inhibition of Arp2\/3, while overexpression restricts lamellipodia formation and limits tumor spread. ARPIN thus acts as a molecular brake on cytoskeletal dynamics and metastatic progression. Research using ARPIN antibody supports studies in cytoskeletal biology, cancer metastasis, and motility regulation.\u003cbr\u003e\u003cbr\u003eARPIN antibody is validated for western blotting, immunofluorescence, and immunohistochemistry to detect actin regulatory proteins. \u003cbr\u003e\u003cbr\u003eStructurally, Actin-related protein complex inhibitor consists of an N-terminal acidic region, a coiled-coil central domain, and a conserved C-terminal WCA-like motif that mediates interaction with Arp2\/3. This domain organization allows ARPIN to act as a direct inhibitor without requiring scaffolding cofactors. This antibody facilitates investigation of ARPIN's role in cytoskeletal remodeling and directional motility across diverse biological systems.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=ARPIN - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=ARPIN - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=ARPIN - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/ARPIN - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=ARPIN+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047319560557,"sku":"FY13238","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_aeab9864-31be-413d-9dbc-bba4fdd0c51c.jpg?v=1782237090","url":"https:\/\/www.ebiohippo.com\/products\/arpin-antibody-actin-related-protein-complex-inhibitor-bha17136140","provider":"BioHippo","version":"1.0","type":"link"}