{"product_id":"ascl1-antibody-achaete-scute-homolog-1-bha17135480","title":"ASCL1 Antibody \/ Achaete-scute homolog 1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eASCL1 Antibody \/ Achaete-scute homolog 1 is a anti-ASCL1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear, cytoplasmic.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e ASCL1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, FACS\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eASCL1 antibody detects Achaete-scute homolog 1, a basic helix-loop-helix (bHLH) transcription factor that directs neuronal differentiation and development. ASCL1 acts as a master regulator of neurogenesis by activating transcriptional programs required for neuronal commitment and inhibiting progenitor cell proliferation. The ASCL1 antibody is widely used in developmental biology, stem cell research, and neuro-oncology to examine neuronal fate specification and transcriptional regulation.\u003cbr\u003e\u003cbr\u003eASCL1 is encoded by the ASCL1 gene on human chromosome 12q23.2. The protein is approximately 194 amino acids long and contains a highly conserved bHLH domain that mediates DNA binding and dimerization with E proteins. ASCL1 recognizes E-box motifs within target gene promoters to activate transcription of neuronal genes, including those involved in axon guidance, neurotransmitter synthesis, and synaptic formation.\u003cbr\u003e\u003cbr\u003eThe ASCL1 antibody detects a 45 kilodalton protein by western blot and exhibits nuclear staining in differentiating neuronal populations. ASCL1 expression peaks during early neurogenesis and declines as cells mature into neurons. It functions in concert with other transcription factors such as NEUROD1 and POU3F2 to coordinate the transition from proliferating progenitors to postmitotic neurons.\u003cbr\u003e\u003cbr\u003eIn addition to its developmental role, ASCL1 is reactivated in neuroendocrine tumors such as small-cell lung carcinoma, where it drives expression of genes promoting proliferation and neuroendocrine identity. ASCL1 also serves as a key transcriptional reprogramming factor used to convert fibroblasts into induced neurons in vitro, illustrating its capacity to initiate neuronal gene networks in non-neural cells.\u003cbr\u003e\u003cbr\u003eBecause ASCL1 integrates differentiation and proliferation control, it serves as a critical model for studying lineage specification and transcriptional reprogramming.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=ASCL1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=ASCL1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=ASCL1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/ASCL1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=ASCL1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047292920173,"sku":"FY12577","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_ea5dc01d-1e02-40df-8700-c3e976869610.jpg?v=1782237025","url":"https:\/\/www.ebiohippo.com\/products\/ascl1-antibody-achaete-scute-homolog-1-bha17135480","provider":"BioHippo","version":"1.0","type":"link"}