{"product_id":"at2-knockout-immortalized-mouse-renal-proximal-tubule-cell-line-bhc10900978","title":"AT2 Knockout Immortalized Mouse Renal Proximal Tubule Cell Line","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eThe proximal tubule is part of the nephron duct system in the kidney, involved in regulating the renin-angiotension system. Cells of this region aid in regulating sodium and volume homeostasis as well as regulating the systemic blood pressure.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eModel identity:\u003c\/strong\u003e AT2 Knockout Immortalized Mouse Renal Proximal Tubule Cell Line is supplied as an engineered cell line derived from Mouse kidney.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Adherent, cobblestone\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth conditions:\u003c\/strong\u003e Use of Applied Cell Extracellular Matrix (G422) is required for cell adhesion and growth. PriGrow IV (TM004) + 5% FBS + 10 nM aldosterone + 50 μM L-ascorbic acid 2-phosphate + 4 μg\/mL dexamethasone + 10 ng\/ml rmEGF (Z200025) + 5 μg\/mL insulin (Z101065) + 20 nM sodium selenite + 5 μg\/mL transferrin + 1 nM T3 + 10 U\/mL IFNG (Z200085) + 1% Penicillin\/Streptomycin Solution (G255), 33.0°C, 5% CO₂\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eEngineering \/ immortalization:\u003c\/strong\u003e Isolated from transgenic mouse (C57BL\/6J x Immortomouse) carrying a temperature-sensitive simian virus 40 tumor antigen (tsA58)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct format:\u003c\/strong\u003e Frozen, BSL-2\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis cell-based model is generally used in immunology, hematology, and signaling studies. Donor\/background information is available for contextual interpretation.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eThe protein Angiotension II (Ang II) and its receptors AT1 and AT2 are some of the most studied proteins in this system. The AT2 Knockout Immortalized Mouse Renal Proximal Tubule Cell Line is a conditionally immortalized mouse renal proximal tubule cells isolated from the mouse harboring thermolabile mutation (tsA58) of the simian virus 40 large T antigen. The functional expression of the SV40 large T antigen is induced by culturing the cells in vitro in medium containing IFN γ at a temperature permissive (33°C). At a non-permissive temperature (37°C-39°C), the cells cease to proliferate. When paired up with the wild type (Cat. No. T0624) and other angiotensin receptor (Ang II)-deficient cell lines AT1A-\/- (Cat. No. T0626), AT1B-\/- (Cat. No. T0627), and AT1A-\/- AT1B-\/- (Cat. No. T0628), these cell lines represent valuable tools to study fluid and electrolyte transportation in the proximal tubule region. RT-PCR was performed to examine products expressed in mutant AT2 -containing cells. Donor\/background information provided for this product: C57BL\/6J x Immortomouse.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eEngineered cell lines are widely used for reporter-based readouts, perturbation studies, and assay optimization in reproducible culture systems.\u003c\/li\u003e\n\u003cli\u003eReporter or transgene-bearing models are often compared with matched parental or control cells to interpret signal changes in context.\u003c\/li\u003e\n\u003cli\u003eExpression trends are typically evaluated alongside passage number, selection pressure, and baseline growth behavior.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eRoutine expansion and maintenance of a defined cell model for downstream in vitro experiments.\u003c\/li\u003e\n\u003cli\u003ePhenotype, signaling, or marker-expression studies performed under standardized culture conditions.\u003c\/li\u003e\n\u003cli\u003eCell-based assay development in which passage number, growth surface, and medium composition are tracked as experimental variables.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eChanges in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMorphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.\u003c\/li\u003e\n\u003cli\u003eMatched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCulture and product details\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e Use of Applied Cell Extracellular Matrix (G422) is required for cell adhesion and growth. PriGrow IV (TM004) + 5% FBS + 10 nM aldosterone + 50 μM L-ascorbic acid 2-phosphate + 4 μg\/mL dexamethasone + 10 ng\/ml rmEGF (Z200025) + 5 μg\/mL insulin (Z101065) + 20 nM sodium selenite + 5 μg\/mL transferrin + 1 nM T3 + 10 U\/mL IFNG (Z200085) + 1% Penicillin\/Streptomycin Solution (G255), 33.0°C, 5% CO₂\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmortalization Method:\u003c\/strong\u003e Isolated from transgenic mouse (C57BL\/6J x Immortomouse) carrying a temperature-sensitive simian virus 40 tumor antigen (tsA58)\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53180514173293,"sku":"T0625","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/YTFsiwBcU6qFI9piHBuV2U4wwcqkDrekRRbbABfZ.png?v=1774957791","url":"https:\/\/www.ebiohippo.com\/products\/at2-knockout-immortalized-mouse-renal-proximal-tubule-cell-line-bhc10900978","provider":"BioHippo","version":"1.0","type":"link"}