{"product_id":"atf5-antibody-activating-transcription-factor-5-bha17135806","title":"ATF5 Antibody \/ Activating transcription factor 5","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eATF5 Antibody \/ Activating transcription factor 5 is a anti-ATF5 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e ATF5\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eATF5 antibody detects Activating transcription factor 5, a stress-responsive transcriptional regulator that modulates cell survival, differentiation, and neural development. Encoded by the ATF5 gene on chromosome 19q13.33, this protein is part of the activating transcription factor\/cAMP response element-binding (ATF\/CREB) family. ATF5 plays key roles in adaptive stress response, neuronal maturation, and maintenance of cellular homeostasis under nutrient deprivation, hypoxia, or endoplasmic reticulum stress.\u003cbr\u003e\u003cbr\u003eStructurally, ATF5 is a 282-amino-acid nuclear protein of approximately 33 kilodaltons containing a basic leucine zipper (bZIP) domain that mediates DNA binding and dimerization with other bZIP family members. Through its N-terminal transactivation domain, ATF5 regulates gene expression involved in apoptosis suppression, differentiation control, and autophagy induction. It is ubiquitously expressed but shows high abundance in neural tissues, liver, and testis, where it participates in differentiation and stress adaptation.\u003cbr\u003e\u003cbr\u003eThe ATF5 antibody is widely used in neuroscience, oncology, and stress physiology research to study transcriptional regulation and survival signaling. Western blot analysis detects a 33 kilodalton band corresponding to ATF5, while immunofluorescence reveals strong nuclear localization and induction under stress conditions. This antibody enables researchers to analyze transcriptional programs governing neurogenesis, stress adaptation, and tumor resistance.\u003cbr\u003e\u003cbr\u003eFunctionally, ATF5 promotes cell survival under stress by inducing anti-apoptotic genes such as BCL2 and HSPA5 while repressing pro-apoptotic transcriptional regulators. In neurons, ATF5 regulates differentiation of neural progenitors and glial cells, influencing brain development and repair. In cancer cells, ATF5 expression confers resistance to nutrient and hypoxia-induced apoptosis, contributing to tumor growth and therapy resistance. The ATF5 antibody provides a reliable reagent for monitoring ATF5 activity in normal and stress-related transcriptional responses.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=ATF5 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=ATF5 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=ATF5 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/ATF5 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=ATF5+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047305470317,"sku":"FY12904","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_30731a07-a3c2-42c4-8d88-e984cc5dab6e.jpg?v=1782237058","url":"https:\/\/www.ebiohippo.com\/products\/atf5-antibody-activating-transcription-factor-5-bha17135806","provider":"BioHippo","version":"1.0","type":"link"}