{"product_id":"atg10-antibody-autophagy-related-protein-10-bha17135539","title":"ATG10 Antibody \/ Autophagy-related protein 10","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eATG10 Antibody \/ Autophagy-related protein 10 is a anti-ATG10 Rabbit antibody Recombinant Rabbit Monoclonal clone ABBD-1 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunoprecipitation (IP) with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e ATG10\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone ABBD-1, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IP\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eATG10 antibody detects Autophagy-related protein 10, an essential E2-like enzyme that catalyzes conjugation of ATG12 to ATG5, a key step in autophagosome formation. ATG10 plays a central role in the autophagy machinery by facilitating complex assembly required for membrane elongation and vesicle maturation. The ATG10 antibody is widely used in cell biology and metabolism research to study autophagy regulation, stress adaptation, and cellular degradation pathways.\u003cbr\u003e\u003cbr\u003eATG10 is encoded by the ATG10 gene located on human chromosome 5q14.1. The protein is approximately 220 amino acids long and resides in the cytoplasm, where it transiently associates with autophagic membranes. Functionally, ATG10 acts as an E2-like enzyme, transferring the ATG12 conjugate to ATG5 under the direction of E1-like enzyme ATG7. This conjugation system forms the ATG12-ATG5-ATG16L1 complex that defines the site of autophagosome formation.\u003cbr\u003e\u003cbr\u003eThe ATG10 antibody detects a 25 kilodalton band by western blot and shows diffuse cytoplasmic staining under confocal microscopy. ATG10 expression increases under nutrient deprivation and oxidative stress, linking metabolic signals to autophagy induction. Inhibition or depletion of ATG10 results in defective autophagosome biogenesis, accumulation of damaged organelles, and increased susceptibility to stress-induced apoptosis.\u003cbr\u003e\u003cbr\u003eATG10 also participates in noncanonical autophagy and immune signaling by modulating antigen presentation and cytokine secretion. Variations in ATG10 have been linked to cancers, inflammatory disorders, and aging-related diseases. Its regulation is tightly controlled through transcriptional activation by FOXO and ATF4, and by post-translational modifications that modulate enzyme activity.\u003cbr\u003e\u003cbr\u003eBecause of its indispensable role in the autophagy conjugation system, ATG10 provides a molecular link between stress sensing and degradative homeostasis.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=ATG10 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=ATG10 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=ATG10 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/ATG10 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=ATG10+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047295148397,"sku":"FY12636","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_08990e7b-0eba-43d4-9bd3-d9eae0793587.jpg?v=1772019322","url":"https:\/\/www.ebiohippo.com\/products\/atg10-antibody-autophagy-related-protein-10-bha17135539","provider":"BioHippo","version":"1.0","type":"link"}