{"product_id":"atp1a2-antibody-atpase-subunit-alpha-2-bha17135948","title":"ATP1A2 Antibody \/ ATPase subunit alpha-2","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eATP1A2 Antibody \/ ATPase subunit alpha-2 is a anti-ATP1A2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e ATP1A2\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eATP1A2 antibody detects Sodium\/potassium-transporting ATPase subunit alpha-2, a membrane enzyme responsible for maintaining electrochemical gradients across the plasma membrane. The UniProt recommended name is Sodium\/potassium-transporting ATPase subunit alpha-2 (ATP1A2). This catalytic subunit is part of the Na+\/K+-ATPase complex, which hydrolyzes ATP to exchange intracellular sodium ions for extracellular potassium ions, a process fundamental to neuronal excitability and muscle contraction.\u003cbr\u003e\u003cbr\u003eFunctionally, ATP1A2 antibody identifies a 1,003-amino-acid integral membrane protein that forms the catalytic core of the Na+\/K+-ATPase. The enzyme functions as a heterodimer composed of an alpha catalytic subunit and a beta regulatory subunit, cycling through phosphorylated and dephosphorylated states to transport ions against their concentration gradients. This active transport maintains membrane potential, osmotic balance, and secondary transport systems that depend on sodium gradients.\u003cbr\u003e\u003cbr\u003eThe ATP1A2 gene is located on chromosome 1q23.2 and encodes the alpha-2 isoform of the Na+\/K+-ATPase, which is predominantly expressed in glial cells, cardiac muscle, and smooth muscle. The alpha-2 isoform differs from the alpha-1 isoform (ATP1A1) in its tissue distribution and kinetic properties, playing a critical role in regulating extracellular potassium buffering and membrane repolarization. In neurons, ATP1A2 contributes to the reuptake of potassium following action potentials, thereby preventing hyperexcitability.\u003cbr\u003e\u003cbr\u003eClinically, mutations in ATP1A2 are associated with familial hemiplegic migraine type 2 (FHM2), alternating hemiplegia of childhood, and other neurological disorders characterized by disturbed ion homeostasis. These mutations can impair ATPase activity, leading to abnormal ionic gradients, cortical spreading depression, and susceptibility to seizures. In cardiac physiology, ATP1A2 supports rhythmic contraction by maintaining proper ion exchange between cytoplasm and extracellular space.\u003cbr\u003e\u003cbr\u003eATP1A2 antibody is widely used in neuroscience, muscle physiology, and ion transport research. It is suitable for immunohistochemistry, western blotting, and immunofluorescence to detect ATP1A2 expression in excitable tissues. This antibody supports studies of membrane transport, neuronal signaling, and electrochemical regulation. In disease research, it aids in evaluating Na+\/K+-ATPase dysfunction in migraine, epilepsy, and cardiovascular disorders.\u003cbr\u003e\u003cbr\u003eStructurally, ATP1A2 contains ten transmembrane helices, a cytoplasmic ATP-binding site, and a phosphorylation domain that drives conformational cycling. It interacts with regulatory proteins including phospholemman and FXYD family members.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=ATP1A2 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=ATP1A2 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=ATP1A2 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/ATP1A2 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=ATP1A2+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047309926765,"sku":"FY13046","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_3e04c6bf-56b2-432c-b963-46cc658f1f1e.jpg?v=1782237070","url":"https:\/\/www.ebiohippo.com\/products\/atp1a2-antibody-atpase-subunit-alpha-2-bha17135948","provider":"BioHippo","version":"1.0","type":"link"}