{"product_id":"atp1a4-antibody-atpase-subunit-alpha-4-bha17135504","title":"ATP1A4 Antibody \/ ATPase subunit alpha 4","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eATP1A4 Antibody \/ ATPase subunit alpha 4 is a anti-ATP1A4 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e ATP1A4\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eATP1A4 antibody detects Sodium\/potassium-transporting ATPase subunit alpha-4, a testis-enriched isoform of the Na+\/K+ ATPase that maintains ionic gradients across the plasma membrane. ATP1A4 catalyzes ATP hydrolysis to drive the active transport of sodium and potassium ions, sustaining electrochemical gradients essential for cell volume regulation, signal transduction, and sperm motility. The ATP1A4 antibody is widely used in reproductive biology, membrane physiology, and enzymology research to study ion homeostasis and energy metabolism.\u003cbr\u003e\u003cbr\u003eATP1A4 is encoded by the ATP1A4 gene located on human chromosome 1p13.1. The protein is approximately 1,023 amino acids long and forms the catalytic alpha subunit of the Na+\/K+ ATPase complex, which also includes a beta subunit and an FXYD regulatory protein. This enzyme maintains intracellular potassium and extracellular sodium concentrations by hydrolyzing ATP for ion exchange across the plasma membrane. The alpha-4 isoform is primarily expressed in spermatozoa and germ cells, where it contributes to flagellar motility and fertility.\u003cbr\u003e\u003cbr\u003eThe ATP1A4 antibody detects a 112 kilodalton band by western blot and shows membrane localization in sperm and epithelial tissues under immunofluorescence microscopy. During spermatogenesis, ATP1A4 supports sperm maturation by regulating intracellular pH and calcium signaling. It localizes predominantly to the sperm plasma membrane, particularly the midpiece and flagellum, providing energy coupling for motility. Functional studies show that loss of ATP1A4 impairs sperm motility, reduces fertility, and alters ionic homeostasis in the reproductive tract.\u003cbr\u003e\u003cbr\u003eIn somatic cells, ATP1A4 contributes to cellular polarization and signal transduction. Beyond ion transport, it functions as a signaling molecule, interacting with Src-family kinases and regulating MAPK pathways during sperm capacitation. This dual role as both a pump and signaling scaffold underscores its importance in reproductive physiology and beyond.\u003cbr\u003e\u003cbr\u003eBecause of its testis-specific expression and regulatory versatility, ATP1A4 serves as a biomarker for sperm function, male fertility, and Na+\/K+ ATPase diversity.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=ATP1A4 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=ATP1A4 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=ATP1A4 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/ATP1A4 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=ATP1A4+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047294034285,"sku":"FY12601","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_f6b5b73f-0cef-49fc-a066-f33e6f962f82.jpg?v=1782237034","url":"https:\/\/www.ebiohippo.com\/products\/atp1a4-antibody-atpase-subunit-alpha-4-bha17135504","provider":"BioHippo","version":"1.0","type":"link"}