{"product_id":"atp5g1-antibody-atp5mc1-bha17135959","title":"ATP5G1 Antibody \/ ATP5MC1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eATP5G1 Antibody \/ ATP5MC1 is a anti-ATP5G1 Rabbit antibody Recombinant Rabbit Monoclonal clone 29A74 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC) with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e ATP5G1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 29A74, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eATP5G1 antibody detects ATP synthase subunit c1, mitochondrial, also called ATP5MC1, encoded by the ATP5G1 gene. This protein is part of the F0 sector of mitochondrial ATP synthase, also known as Complex V of the oxidative phosphorylation system. ATP5G1 contributes to the proton translocation channel that drives rotary catalysis, coupling the proton gradient to ATP production. ATP5G1 antibody provides researchers with a powerful tool to study mitochondrial bioenergetics, oxidative phosphorylation, and metabolic disease.\u003cbr\u003e\u003cbr\u003eATP synthase consists of two major domains: the F1 catalytic domain, which synthesizes ATP from ADP and inorganic phosphate, and the F0 membrane domain, which translocates protons across the inner mitochondrial membrane. ATP5G1 is one of the small c subunits that form the proton conducting ring of the F0 domain. Research using ATP5G1 antibody has revealed that this protein ensures efficient proton transport and rotary motion, allowing the enzyme to generate ATP that fuels cellular metabolism. Disruption of this subunit destabilizes ATP synthase and reduces energy production.\u003cbr\u003e\u003cbr\u003eMutations in ATP5G1 and related subunits have been associated with mitochondrial diseases, including encephalomyopathy and metabolic syndromes. Studies with ATP5G1 antibody have confirmed that defects impair ATP production and contribute to developmental delay, neuromuscular symptoms, and metabolic acidosis. Because ATP synthase is fundamental for energy metabolism, ATP5G1 remains essential for tissue homeostasis in high energy demanding organs such as brain, muscle, and heart.\u003cbr\u003e\u003cbr\u003eIn addition to inherited disorders, mitochondrial dysfunction involving ATP synthase subunits contributes to aging, neurodegeneration, and cancer. Research using ATP5G1 antibody has demonstrated altered expression in tumors, where shifts in energy metabolism favor glycolysis over oxidative phosphorylation. In neurons, reduced ATP synthase activity contributes to synaptic dysfunction and neurodegeneration. These findings highlight the broad significance of ATP5G1 in health and disease.\u003cbr\u003e\u003cbr\u003eATP5G1 antibody is widely used in western blotting, immunohistochemistry, and immunofluorescence. Western blotting demonstrates abundance in mitochondria rich tissues, immunohistochemistry highlights distribution in heart, muscle, and brain, and immunofluorescence reveals localization to mitochondrial networks. Together, these methods establish ATP5G1 antibody as a core tool for mitochondrial research.\u003cbr\u003e\u003cbr\u003eBy providing validated ATP5G1 antibody reagents,\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=ATP5G1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=ATP5G1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=ATP5G1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/ATP5G1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=ATP5G1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047310778733,"sku":"FY13057","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_66c14a47-cde9-41c4-9893-6fdbbe620603.jpg?v=1772019384","url":"https:\/\/www.ebiohippo.com\/products\/atp5g1-antibody-atp5mc1-bha17135959","provider":"BioHippo","version":"1.0","type":"link"}