{"product_id":"atp6v0a1-antibody-biotin-conjugated-bha10528919","title":"ATP6V0A1 Antibody, Biotin conjugated","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eThis polyclonal anti-ATP6V0A1 antibody raised in Rabbit, conjugated with Biotin, tested in ELISA. Designed to detect ATP6V0A1 in Human for others research.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunogen:\u003c\/strong\u003e Recombinant Human V-type proton ATPase 116 kDa subunit a isoform 1 protein (80-260AA) — determines epitope; confirm sample prep compatibility.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHost (Rabbit):\u003c\/strong\u003e Requires anti-rabbit-IgG secondary matching isotype IgG.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePolyclonal:\u003c\/strong\u003e Multiple epitopes = robust signal; lot-to-lot variability — include controls.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eIsotype (IgG):\u003c\/strong\u003e Match secondary, Protein A\/G, and isotype controls to IgG.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurification (Protein G purification):\u003c\/strong\u003e Enriches Ig classes; reduces non-specific background.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBiotin:\u003c\/strong\u003e Streptavidin\/avidin amplification; block endogenous biotin in biotin-rich samples.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cp\u003e\u003cstrong\u003eATP6V0A1\u003c\/strong\u003e (also: a1, ATP6N1, ATP6N1A, ATP6V0A1, ATPase H+ transporting lysosomal (vacuolar proton pump) non catalytic accessory protein 1A (110\/116kD), ATPase H+ transporting lysosomal non catalytic accessory protein 1 (110\/116kD), ATPase H+ transporting lysosomal V0 subunit a1, ATPase H+ transporting V0 subunit a1, ATPase, H+ transporting, lysosomal, noncatalytic accessory protein 1A, Clathrin coated vesicle\/synaptic vesicle proton pump 116 kDa subunit, Clathrin-coated vesicle\/synaptic vesicle proton pump 116 kDa subunit, DKFZp781J1951, H(+) transporting two sector ATPase 116 kDa accessory protein A1, Stv1, V ATPase 116 kDa, V ATPase 116 kDa isoform a1, V type proton ATPase 116 kDa subunit a, V type proton ATPase 116 kDa subunit a isoform 1, V-ATPase 116 kDa isoform a1, V-type proton ATPase 116 kDa subunit a isoform 1, Vacuolar adenosine triphosphatase subunit Ac116, Vacuolar proton pump subunit 1, Vacuolar proton pump, subunit 1, Vacuolar proton translocating ATPase 116 kDa subunit A, Vacuolar proton translocating ATPase 116 kDa subunit a isoform 1, Vacuolar type H(+) ATPase 115 kDa subunit, Vph1, VPP1, VPP1_HUMAN) — studied in Human systems. Expression, localization, and PTMs vary across cell types and disease states. Consult UniProt, NCBI Gene, and primary literature for current ATP6V0A1 annotation relevant to others.\u003c\/p\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e Quantify soluble target; ensure samples within the linear detection range.\u003c\/li\u003e\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eIsotype controls:\u003c\/strong\u003e IgG from Rabbit at equivalent concentration.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCross-reactivity:\u003c\/strong\u003e Polyclonal: validate with KO\/KD controls.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMatrix effects:\u003c\/strong\u003e Verify dilution linearity in your sample matrix.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReactivity:\u003c\/strong\u003e Confirmed for Human; extrapolation requires validation.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cem\u003eThis product is a polyclonal antibody purified from rabbit antiserum.\u003c\/em\u003e\u003c\/p\u003e\u003c!-- Sources: UniProt, NCBI Gene, Cusabio, Human Protein Atlas, PubMed --\u003e","brand":"CUSABIO TECHNOLOGY LLC","offers":[{"title":"50 ug","offer_id":53240841929069,"sku":"CSB-PA846292LD01HU-50UG","price":170.98,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53240943608173,"sku":"CSB-PA846292LD01HU-100UG","price":307.97,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/Cusabio_Antibody_Placeholder_da278aea-b81b-45da-9adf-ecdd79ed02ef.jpg?v=1784926920","url":"https:\/\/www.ebiohippo.com\/products\/atp6v0a1-antibody-biotin-conjugated-bha10528919","provider":"BioHippo","version":"1.0","type":"link"}