{"product_id":"atp6v1b2-antibody-atpase-h-and-transporting-v1-subunit-b2-bha17135582","title":"ATP6V1B2 Antibody \/ ATPase H+ transporting V1 subunit B2","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eATP6V1B2 Antibody \/ ATPase H+ transporting V1 subunit B2 is a anti-ATP6V1B2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e ATP6V1B2\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eATP6V1B2 antibody recognizes ATPase H+ transporting V1 subunit B2, a catalytic subunit of the vacuolar ATPase (V-ATPase) complex that mediates proton translocation across intracellular membranes. V-ATPases are multi-subunit enzymes responsible for acidifying organelles such as endosomes, lysosomes, the Golgi apparatus, and synaptic vesicles, thereby regulating protein sorting, receptor recycling, and neurotransmitter loading. The B2 subunit encoded by the ATP6V1B2 gene provides ATP-hydrolytic activity within the V1 sector of the complex, coupling energy derived from ATP hydrolysis to proton pumping through the V0 membrane domain. This process maintains intracellular pH homeostasis, a prerequisite for vesicular trafficking, autophagy, and enzymatic activity within acidic compartments.\u003cbr\u003e\u003cbr\u003eATP6V1B2 is highly expressed in neural tissues, particularly in neurons where acidification of synaptic vesicles supports neurotransmitter storage and release. The protein localizes to the cytoplasmic side of vesicular membranes and interacts with other V1 subunits including A, D, and E to form the catalytic hexameric headpiece. Genetic studies have linked mutations in ATP6V1B2 to dominant deafness-onychodystrophy syndrome, characterized by hearing loss and nail dysplasia, and to developmental disorders with epilepsy, underscoring its physiological importance in neural and epithelial systems. By sustaining organelle acidification, ATP6V1B2 ensures correct maturation of lysosomal enzymes and degradation of endocytosed material, influencing neuronal viability and metabolic turnover.\u003cbr\u003e\u003cbr\u003eThe ATP6V1B2 antibody is used in neuroscience, cell biology, and pathology research to examine the localization and function of V-ATPase complexes. Immunofluorescence and immunohistochemistry reveal strong punctate staining in synaptic regions, consistent with the protein's vesicular distribution. In biochemical assays, western blot detection with this antibody confirms the ~56 kilodalton B2 subunit and can distinguish it from the related B1 isoform (ATP6V1B1) found mainly in kidney and inner ear epithelia. Because of its neural enrichment, ATP6V1B2 serves as a marker for vesicle acidification and proton pump activity in brain tissue and cultured neurons.\u003cbr\u003e\u003cbr\u003eResearch indicates that ATP6V1B2 also participates in cellular signaling and autophagy. V-ATPase activity influences mTORC1 localization and nutrient-sensing pathways by regulating lysosomal pH. Deficiency of ATP6V1B2 disrupts these mechanisms, impairing cell growth and autophagic flux. The antibody thus enables studies investigating the intersection of metabolism, membrane transport, and neurodegenerative disease. Alterations in V-ATPase function have been observed in Alzheimer's and Parkinson's disease models, making ATP6V1B2 a potential biomarker for vesicular dysfunction.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=ATP6V1B2 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=ATP6V1B2 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=ATP6V1B2 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/ATP6V1B2 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=ATP6V1B2+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047296328045,"sku":"FY12679","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_0e129070-2826-45fa-8956-384777057172.jpg?v=1782237028","url":"https:\/\/www.ebiohippo.com\/products\/atp6v1b2-antibody-atpase-h-and-transporting-v1-subunit-b2-bha17135582","provider":"BioHippo","version":"1.0","type":"link"}