{"product_id":"atp7a-antibody-copper-transporting-atpase-1-bha17135305","title":"ATP7A Antibody \/ Copper-transporting ATPase 1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eATP7A Antibody \/ Copper-transporting ATPase 1 is a anti-ATP7A Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS) with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e ATP7A\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eThe ATP7A antibody targets Copper-transporting ATPase 1, a P-type ATPase encoded by the ATP7A gene that regulates intracellular copper distribution and homeostasis. Copper-transporting ATPase 1 facilitates the delivery of copper to cuproenzymes within the trans-Golgi network and mediates copper efflux from cells when intracellular levels rise. The ATP7A antibody provides a robust tool for investigating copper metabolism, metal ion transport, and disorders of copper imbalance such as Menkes disease.\u003cbr\u003e\u003cbr\u003eCopper-transporting ATPase 1 is a multi-domain membrane protein containing ATP-binding and transmembrane copper-binding motifs that drive active ion transport using ATP hydrolysis. It localizes mainly to the trans-Golgi network under basal conditions but relocates to the plasma membrane during copper overload. The ATP7A antibody allows researchers to study this dynamic localization, revealing how cells adapt to fluctuating copper availability to maintain metal homeostasis. ATP7A activity ensures proper metallation of key enzymes such as lysyl oxidase, dopamine beta-hydroxylase, and superoxide dismutase 1.\u003cbr\u003e\u003cbr\u003eLoss-of-function mutations in ATP7A cause Menkes disease, a lethal X-linked disorder characterized by systemic copper deficiency leading to neurodegeneration, connective tissue abnormalities, and growth retardation. The ATP7A antibody is crucial for diagnostic and mechanistic research into this condition, enabling detection of protein expression and distribution defects in patient-derived fibroblasts and tissues. Reduced expression or mislocalization of Copper-transporting ATPase 1 disrupts copper delivery to secretory enzymes, impairing multiple physiological processes.\u003cbr\u003e\u003cbr\u003eIn addition to Menkes disease, partial ATP7A dysfunction underlies occipital horn syndrome, a milder disorder affecting connective tissue and autonomic function. The ATP7A antibody supports comparative studies of these phenotypes and aids in identifying molecular defects in copper trafficking. Furthermore, ATP7A expression influences tumor progression and drug resistance, as copper transport impacts angiogenesis and the uptake of platinum-based chemotherapeutics.\u003cbr\u003e\u003cbr\u003eThe ATP7A antibody is validated for western blotting, immunohistochemistry, and immunofluorescence, showing clear perinuclear and membrane localization depending on copper conditions.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=ATP7A - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=ATP7A - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=ATP7A - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/ATP7A - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=ATP7A+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047287972205,"sku":"FY12402","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_27e2d887-c0d6-4eda-9369-57c858440b77.jpg?v=1782237006","url":"https:\/\/www.ebiohippo.com\/products\/atp7a-antibody-copper-transporting-atpase-1-bha17135305","provider":"BioHippo","version":"1.0","type":"link"}