{"product_id":"axin1-antibody-axin-1-bha17136050","title":"AXIN1 Antibody \/ Axin 1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eAXIN1 Antibody \/ Axin 1 is a anti-AXIN1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA with listed reactivity in Human, Mouse. Reported localization: Nuclear, cytoplasmic.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e AXIN1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC, IF, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eAXIN1 antibody detects Axin-1, a scaffolding protein that regulates Wnt signaling and beta-catenin degradation. The UniProt recommended name is Axin-1 (AXIN1). This cytoplasmic protein serves as a core component of the beta-catenin destruction complex, coordinating phosphorylation and proteasomal turnover of beta-catenin to suppress Wnt pathway activation.\u003cbr\u003e\u003cbr\u003eFunctionally, AXIN1 antibody identifies a 862-amino-acid protein containing a regulator of G protein signaling (RGS) domain, a beta-catenin-binding region, and a DIX domain for homodimerization. AXIN1 recruits APC, GSK3B, and CK1 to form a multiprotein complex that phosphorylates beta-catenin, marking it for degradation. This mechanism ensures tight control of cell proliferation, polarity, and differentiation.\u003cbr\u003e\u003cbr\u003eThe AXIN1 gene is located on chromosome 16p13.3 and is expressed in most tissues, particularly in epithelial and neural cells. As a negative regulator of Wnt signaling, AXIN1 maintains developmental patterning and prevents aberrant activation of oncogenic transcription programs.\u003cbr\u003e\u003cbr\u003ePathologically, loss or mutation of AXIN1 is associated with hepatocellular carcinoma, colorectal cancer, and developmental anomalies. Decreased AXIN1 function stabilizes beta-catenin, promoting tumor growth and metastasis. Research using AXIN1 antibody supports studies in Wnt pathway regulation, cancer biology, and cell signaling control.\u003cbr\u003e\u003cbr\u003eAXIN1 antibody is validated for western blotting, immunohistochemistry, and immunofluorescence to detect Wnt signaling regulators and cytoskeletal scaffolding proteins. \u003cbr\u003e\u003cbr\u003eStructurally, Axin-1 forms extended multimolecular complexes through its DIX domain and interacts with multiple kinases and phosphatases. This antibody enables analysis of AXIN1's scaffold-dependent regulation of beta-catenin turnover and pathway suppression.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=AXIN1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=AXIN1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=AXIN1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/AXIN1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=AXIN1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047313596781,"sku":"FY13148","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_81737156-83b4-44ed-8b6a-39beb66b16a6.jpg?v=1782237089","url":"https:\/\/www.ebiohippo.com\/products\/axin1-antibody-axin-1-bha17136050","provider":"BioHippo","version":"1.0","type":"link"}