{"product_id":"b7-2-cd86-orf-cdna-lentivirus-bhv19400113","title":"B7-2 (CD86) ORF cDNA Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eB7-2 (CD86) is a co-stimulatory glycoprotein expressed on the surface of antigen-presenting cells such as dendritic cells, macrophages, and B cells. By binding the receptors CD28 and CTLA-4 on T cells, CD86 delivers the second signal required for full T cell activation: engagement of CD28 promotes proliferation and cytokine production, whereas engagement of CTLA-4 transmits inhibitory signals. CD86 therefore helps set the balance between T cell activation and tolerance, and its expression is upregulated during immune responses. Because of its central role in T cell priming, CD86 is an important molecule in immunology, vaccine research, and the study of immune checkpoint pathways.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe B7-2 (CD86) ORF cDNA Lentivirus delivers lentiviral particles packaged with an expression vector for human or mouse CD86, with multiple transcript variants available. A CMV or EF1a promoter drives the ORF cDNA fused to a C-terminal epitope tag, while a fluorescent reporter (GFP or RFP) and drug-selection marker (puromycin or blasticidin) are separated by a self-cleaving peptide for independent expression from the same transcript; in some constructs the reporter and marker are driven separately by a PGK promoter. ORF cloning accuracy has been confirmed by sequencing and protein expression validated by transient transfection. The vectors provide high, stable CD86 expression and reliable genome integration, enabling long-term stable cell lines. They efficiently transduce difficult-to-transfect cells, including primary and thawed cells, for studies of T cell co-stimulation and immune function.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis ORF cDNA lentivirus enables stable overexpression of B7-2 (CD86) (NCBI Accession: NM_019388, NM_175862) in mammalian cells via a third-generation, VSV-G pseudotyped delivery system. The ORF cDNA is fused to a C-terminal epitope tag (V5, Myc, or HA) and expressed under a strong constitutive promoter (CMV). Reporter and selection marker components (GFP, RFP; Blasticidin, Puromycin) are co-expressed via self-cleaving P2A peptides, enabling independent protein production without fusion-tag artifacts.\u003c\/p\u003e\n\u003cp\u003eUltra-purification by PEG precipitation and sucrose gradient centrifugation yields high-titer particles suitable for primary cells, suspension cultures, and stem cells. Stable polyclonal cell lines are established within 10–14 days by antibiotic selection or FACS sorting. For in vivo applications, the serum-free formulation and VSV-G envelope support direct administration or further concentration for stereotactic injection.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"CMV-Cd86-V5 \/ RFP\/none \/ 5x10^6","offer_id":53251619815789,"sku":"LCV-0021-6","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-Cd86-V5 \/ GFP\/none \/ 5x10^6","offer_id":53311253381485,"sku":"LCV-0021-5","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-B7-2-V5 \/ RFP\/none \/ 5x10^6","offer_id":53311253414253,"sku":"LCV-0021-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-B7-2-V5 \/ GFP\/none \/ 5x10^6","offer_id":53311253447021,"sku":"LCV-0021-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-B7-2-Flag \/ none\/Puromycin \/ 5x10^6","offer_id":53311253479789,"sku":"LCV-0021-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-B7-2-Flag \/ none\/Blasticidin \/ 5x10^6","offer_id":53311253512557,"sku":"LCV-0021-4S","price":595.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/www.ebiohippo.com\/products\/b7-2-cd86-orf-cdna-lentivirus-bhv19400113","provider":"BioHippo","version":"1.0","type":"link"}