{"product_id":"bacterial-dna-extraction-kit-bht15200017","title":"Bacterial DNA Extraction Kit","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-license-box{background:#f5f5f5;border:1px solid #ddd;border-radius:4px;padding:12px 16px;font-size:14px;color:#444}.bhp-desc .bhp-regulatory-row{display:flex;gap:16px;flex-wrap:wrap;margin:6px 0 10px}.bhp-desc .bhp-reg-item{background:#f0f6ff;border:1px solid #ccd9f0;border-radius:4px;padding:6px 12px;font-size:14px}.bhp-desc .bhp-reg-item strong{color:#003366;display:block;font-size:12px;text-transform:uppercase;letter-spacing:.5px}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}.bhp-desc .bhp-comp-table th{width:auto}\u003c\/style\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eDNA Extraction Kits\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eThis product is suitable for the separation and purification of total DNA from 1–5 mL bacterial culture. After the bacteria are broken by lysozyme, they are dissolved by Buffer BL-1, and then precipitated by Buffer BL-2 to remove protein and cell debris. The genomic DNA in the centrifugal supernatant can be bound to the purification column. After washing with Buffer RP and Buffer WB to remove the proteins and PCR inhibitors remaining on the membrane, the genomic DNA is eluted with Buffer TE and can be used in various molecular biology experiments immediately.\u003c\/p\u003e\n\u003cp\u003eThe bacterial pellet is resuspended in 200 µL of Buffer TE, digested with 100 µL of lysozyme solution at 37°C for 30–60 min, then treated with 225 µL of Buffer BL-1 and 225 µL of Buffer BL-2 with 30 s of vortexing each. After clarification at 13,000 rpm for 2 min the supernatant is bound to the purification column, washed with 500 µL of Buffer RP and 600 µL of Buffer WB, dried at 14,000 rpm for 1 min and eluted with 50–100 µL of warm Buffer TE. The OD260\/OD280 ratio should be 1.7–1.9.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eFormat\u003c\/th\u003e\n\u003ctd\u003eSpin column (nucleic acid purification column)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSample type\u003c\/th\u003e\n\u003ctd\u003eBacterial culture\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSample input\u003c\/th\u003e\n\u003ctd\u003e1–5 mL bacterial culture\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurity (OD260\/OD280)\u003c\/th\u003e\n\u003ctd\u003e1.7–1.9\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eElution volume\u003c\/th\u003e\n\u003ctd\u003e50–100 µL Buffer TE at 37°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eProcessing time\u003c\/th\u003e\n\u003ctd\u003eWithin 1 hour for Gram-negative bacteria\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecial treatment\u003c\/th\u003e\n\u003ctd\u003eLysozyme supplied; no proteinase K or RNase digestion step required\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table bhp-comp-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eComponent\u003c\/th\u003e\n\u003cth\u003e50 T\u003c\/th\u003e\n\u003cth\u003e200 T\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer BL-1\u003c\/td\u003e\n\u003ctd\u003e15 mL\u003c\/td\u003e\n\u003ctd\u003e60 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer BL-2\u003c\/td\u003e\n\u003ctd\u003e15 mL\u003c\/td\u003e\n\u003ctd\u003e60 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer RP\u003c\/td\u003e\n\u003ctd\u003e30 mL\u003c\/td\u003e\n\u003ctd\u003e120 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eLysozyme\u003c\/td\u003e\n\u003ctd\u003e600 mg\u003c\/td\u003e\n\u003ctd\u003e2,400 mg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer WB\u003c\/td\u003e\n\u003ctd\u003e40 mL\u003c\/td\u003e\n\u003ctd\u003e160 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer TE\u003c\/td\u003e\n\u003ctd\u003e15 mL\u003c\/td\u003e\n\u003ctd\u003e60 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eNucleic acid purification column\u003c\/td\u003e\n\u003ctd\u003e50 set\u003c\/td\u003e\n\u003ctd\u003e200 set\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eTotal DNA extraction from bacterial culture\u003c\/li\u003e\n\u003cli\u003eGenomic DNA for PCR\u003c\/li\u003e\n\u003cli\u003eGenomic DNA for enzyme digestion\u003c\/li\u003e\n\u003cli\u003eGenomic DNA for hybridization\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eKey Features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eSimple and fast: Gram-negative bacteria can obtain high-quality genomic DNA within 1 hour.\u003c\/li\u003e\n\u003cli\u003eNo proteinase K or RNase digestion step is required.\u003c\/li\u003e\n\u003cli\u003eGood quality: DNA can be directly used in molecular biology experiments such as PCR, enzyme digestion, and hybridization.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eStorage \u0026amp; Handling\u003c\/h2\u003e\n\u003cp\u003eBuffers BL-1, BL-2, RP, WB and TE and the purification columns are stored at room temperature for 1 year. Lysozyme is stored at -20°C for 1 year.\u003c\/p\u003e\n\u003ch2\u003eUsage Notes\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eAvoid repeated freeze-thaw cycles.\u003c\/li\u003e\n\u003cli\u003eConfirm that absolute ethanol has been added to the wash buffers before first use.\u003c\/li\u003e\n\u003cli\u003eThick-walled bacterial species require a longer lysozyme incubation.\u003c\/li\u003e\n\u003cli\u003eRNA can be removed by an optional RNase A treatment at 1:200.\u003c\/li\u003e\n\u003cli\u003eEnsure complete lysis to avoid blocking the column.\u003c\/li\u003e\n\u003cli\u003eStore the eluted DNA at -20°C.\u003c\/li\u003e\n\u003cli\u003eLysozyme is supplied as 600 mg (50 T) or 2,400 mg (200 T); the protocol uses 100 µL of lysozyme solution per preparation. Follow the manufacturer's manual for its preparation.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSafety \u0026amp; Regulatory\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp\u003eFor research use only. Not intended for clinical, diagnostic or therapeutic use. Follow the manufacturer's manual and standard laboratory safety practice.\u003c\/p\u003e\u003c\/div\u003e\n\u003cdiv class=\"bhp-regulatory-row\"\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eGrade\u003c\/strong\u003eResearch Use Only\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eManufacturer\u003c\/strong\u003eELK Biotechnology\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eCatalog No.\u003c\/strong\u003eEP021\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eBioHippo SKU\u003c\/strong\u003eBHT15200017\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"ELK Biotechnology","offers":[{"title":"50 T","offer_id":53965512606061,"sku":"EP021-50T","price":208.0,"currency_code":"USD","in_stock":true},{"title":"200 T","offer_id":53965514670445,"sku":"EP021-200T","price":390.0,"currency_code":"USD","in_stock":true},{"title":"50 T x 10","offer_id":53965514703213,"sku":"EP021-50TX10","price":0.0,"currency_code":"USD","in_stock":true},{"title":"200 T x 5","offer_id":53965514735981,"sku":"EP021-200TX5","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/EP021-a-1.jpg?v=1789419119","url":"https:\/\/www.ebiohippo.com\/products\/bacterial-dna-extraction-kit-bht15200017","provider":"BioHippo","version":"1.0","type":"link"}