{"product_id":"bcam-recombinant-monoclonal-antibody-bha10576744","title":"BCAM Recombinant Monoclonal Antibody","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eThis is a monoclonal anti-BCAM antibody raised in Rabbit, with confirmed utility in ELISA, IHC. It is designed to detect BCAM protein in Human and supports researchers working in others contexts.\u003c\/p\u003e\n\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunogen:\u003c\/strong\u003e A synthesized peptide from human BCAM protein — determines the epitope region; confirm compatibility with sample preparation and expected post-translational modifications.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHost species (Rabbit):\u003c\/strong\u003e Requires anti-rabbit-IgG secondary reagents for indirect detection.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMonoclonal format:\u003c\/strong\u003e Single-epitope binding provides high specificity and lot-to-lot reproducibility. Confirm epitope accessibility under your assay conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eIsotype (IgG):\u003c\/strong\u003e Matched secondary antibodies and isotype controls required. Compatible with standard Protein A\/G purification workflows.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurification (Affinity chromatography):\u003c\/strong\u003e Enriches for specific immunoglobulin classes; reduces non-specific background vs. crude antisera.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eBCAM\u003c\/strong\u003e (also referred to as Antigen identified by monoclonal antibody F8  antibody, AU antibody, Auberger B antigen antibody, B CAM cell surface glycoprotein antibody, B cell adhesion molecule antibody, B-CAM cell surface glycoprotein antibody, Basal cell adhesion molecule (Lu and Au blood groups) antibody, Basal cell adhesion molecule (Lutheran blood group) antibody, Basal cell adhesion molecule antibody, Basal cell adhesion molecule Lu and Au blood groups antibody, Basal cell adhesion molecule Lutheran blood group antibody, Bcam antibody, BCAM_HUMAN antibody, CD239 antibody, CD239 antigen antibody, F8\/G253 antigen antibody, Glycoprotein 95kDa antibody, LU antibody, Lutheran antibody, Lutheran antigen antibody, Lutheran blood group (Auberger b antigen included) antibody, Lutheran blood group Auberger b antigen included  antibody, Lutheran blood group glycoprotein antibody, MSK19  antibody) is a protein target studied in Human systems. Expression, subcellular localization, and post-translational modifications vary across cell types and disease states — factors critical to antibody-based detection design. Consult UniProt, NCBI Gene, and primary literature for current annotation of BCAM biology in others.\u003c\/p\u003e\n\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e Quantification of soluble target in biological fluids or culture supernatants. Ensure samples are within the linear detection range.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eIHC:\u003c\/strong\u003e Visualization of target in FFPE or frozen tissue sections. Optimize antigen retrieval and secondary detection for each tissue type.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eIsotype controls:\u003c\/strong\u003e Use an isotype-matched (IgG from Rabbit) control at equivalent concentration to assess non-specific background.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCross-reactivity:\u003c\/strong\u003e Confirm the clone does not cross-react with related family members under your assay conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMatrix effects:\u003c\/strong\u003e Sample matrix can affect performance; pilot dilution linearity and spike-recovery experiments are advised for quantitative studies.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSpecies reactivity:\u003c\/strong\u003e Confirmed for Human. Extrapolation to untested species requires empirical validation.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003cp\u003e\u003cem\u003eThis product is a recombinant monoclonal antibody expressed in HEK293F cells.\u003c\/em\u003e\u003c\/p\u003e\n\n\u003c!-- Sources:\n- UniProt: https:\/\/www.uniprot.org\n- NCBI Gene: https:\/\/www.ncbi.nlm.nih.gov\/gene\n- Cusabio: https:\/\/www.cusabio.com\n- Human Protein Atlas: https:\/\/www.proteinatlas.org\n- PubMed: https:\/\/pubmed.ncbi.nlm.nih.gov\n--\u003e","brand":"CUSABIO TECHNOLOGY LLC","offers":[{"title":"50 uL","offer_id":53248428278125,"sku":"CSB-RA958435A0HU-50UL","price":210.0,"currency_code":"USD","in_stock":true},{"title":"100 uL","offer_id":53248502038893,"sku":"CSB-RA958435A0HU-100UL","price":350.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/CSB-RA958435A0HU-IHC-1.jpg?v=1776796045","url":"https:\/\/www.ebiohippo.com\/products\/bcam-recombinant-monoclonal-antibody-bha10576744","provider":"BioHippo","version":"1.0","type":"link"}