{"product_id":"bcar4-antibody-breast-cancer-anti-estrogen-resistance-protein-4-bha17135956","title":"BCAR4 Antibody \/ Breast cancer anti-estrogen resistance protein 4","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eBCAR4 Antibody \/ Breast cancer anti-estrogen resistance protein 4 is a anti-BCAR4 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e BCAR4\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eBCAR4 antibody detects Breast cancer anti-estrogen resistance protein 4, a long non-coding RNA-associated regulatory protein implicated in tumor progression and transcriptional signaling. The UniProt recommended name is Breast cancer anti-estrogen resistance protein 4 (BCAR4). Although originally identified for its role in tamoxifen resistance, BCAR4 has since been recognized as a multifunctional regulator that coordinates growth factor and developmental signaling pathways in both normal and cancerous tissues.\u003cbr\u003e\u003cbr\u003eFunctionally, BCAR4 antibody identifies a small regulatory protein and lncRNA complex component that modulates noncanonical Hedgehog signaling. BCAR4 interacts with transcriptional coactivators GLI2 and SMO, promoting activation of target genes linked to proliferation, migration, and survival. Its expression in breast, lung, and cervical carcinomas correlates with tumor aggressiveness and metastatic potential, where it facilitates estrogen-independent growth and resistance to anti-estrogen therapies.\u003cbr\u003e\u003cbr\u003eThe BCAR4 gene is located on chromosome 16p13.13 and encodes a non-coding transcript that exerts protein-like regulatory effects through RNA-protein interactions. Functionally, BCAR4 acts as a scaffold linking SNIP1 and PNUTS to p300 histone acetyltransferase, thereby enhancing transcriptional activation of downstream effectors involved in cytoskeletal dynamics and metabolic adaptation. In breast cancer, BCAR4-driven signaling enhances glucose metabolism and EMT, supporting invasive phenotypes.\u003cbr\u003e\u003cbr\u003eBeyond oncology, BCAR4 is expressed in developmental tissues, including the embryonic brain and reproductive organs, suggesting functions in morphogenesis and differentiation. Experimental silencing of BCAR4 inhibits cell proliferation and migration, underscoring its essential role in oncogenic signaling cascades. Because of its RNA-protein hybrid function, BCAR4 exemplifies a growing class of noncoding RNAs with direct regulatory influence over gene expression.\u003cbr\u003e\u003cbr\u003eBCAR4 antibody is widely used in cancer biology, gene regulation, and lncRNA research. It is suitable for immunohistochemistry, immunofluorescence, and western blotting to detect BCAR4 protein expression and localization. This antibody supports investigations into transcriptional signaling, drug resistance mechanisms, and cellular differentiation. In translational research, BCAR4 detection assists in studying hormone therapy resistance and metastasis formation.\u003cbr\u003e\u003cbr\u003eStructurally, BCAR4 contains regulatory motifs facilitating interaction with transcriptional cofactors and signaling kinases. Its activity is regulated by chromatin context and nuclear-cytoplasmic shuttling.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=BCAR4 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=BCAR4 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=BCAR4 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/BCAR4 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=BCAR4+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047310975341,"sku":"FY13054","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_7d592010-08e6-4ca4-bcda-7abeff2b666f.jpg?v=1782237104","url":"https:\/\/www.ebiohippo.com\/products\/bcar4-antibody-breast-cancer-anti-estrogen-resistance-protein-4-bha17135956","provider":"BioHippo","version":"1.0","type":"link"}