{"product_id":"bccip-antibody-brca2-and-cdkn1a-interacting-protein-bha17135735","title":"BCCIP Antibody \/ BRCA2 and CDKN1A-interacting protein","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eBCCIP Antibody \/ BRCA2 and CDKN1A-interacting protein is a anti-BCCIP Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e BCCIP\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC, IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eBCCIP antibody detects BRCA2 and CDKN1A-interacting protein, a nuclear protein involved in DNA repair, cell cycle regulation, and genome maintenance. Encoded by the BCCIP gene on chromosome 10q26.2, this protein interacts with BRCA2 and p21 (CDKN1A) to facilitate homologous recombination and checkpoint control. BCCIP functions as a cofactor in the BRCA2-RAD51 repair complex, promoting the repair of double-strand breaks and ensuring replication fork stability during DNA synthesis.\u003cbr\u003e\u003cbr\u003eStructurally, BCCIP contains conserved coiled-coil domains and an acidic region that mediate protein-protein interactions with DNA repair and replication factors. It is expressed in proliferating cells and localizes primarily to the nucleus, particularly at sites of DNA damage. Through its association with p21, BCCIP also influences CDK activity and contributes to G1\/S and G2\/M checkpoint control.\u003cbr\u003e\u003cbr\u003eThe BCCIP antibody is widely used in DNA repair, oncology, and cell cycle research to study BRCA2-associated homologous recombination and genomic integrity. Western blot analysis identifies a 36 kilodalton band corresponding to BCCIP, while immunofluorescence shows nuclear punctate staining consistent with DNA damage foci. This antibody enables detection of repair complex dynamics and checkpoint activation in mammalian cells.\u003cbr\u003e\u003cbr\u003eDysregulation of BCCIP expression impairs DNA repair fidelity, leading to chromosomal instability, replication stress, and increased tumor susceptibility. Reduced BCCIP levels have been observed in glioblastoma and colorectal cancer, supporting its role as a tumor suppressor. The BCCIP antibody provides a reliable reagent for exploring DNA damage response and tumorigenic mechanisms.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=BCCIP - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=BCCIP - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=BCCIP - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/BCCIP - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=BCCIP+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047303995757,"sku":"FY12833","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_4f673834-ab95-4f3f-ae8b-29a6818747da.jpg?v=1782237060","url":"https:\/\/www.ebiohippo.com\/products\/bccip-antibody-brca2-and-cdkn1a-interacting-protein-bha17135735","provider":"BioHippo","version":"1.0","type":"link"}