{"product_id":"bche-antibody-butyrylcholinesterase-bha17135928","title":"BCHE Antibody \/ Butyrylcholinesterase","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eBCHE Antibody \/ Butyrylcholinesterase is a anti-BCHE Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e BCHE\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eBCHE antibody detects Butyrylcholinesterase, a serine hydrolase that catalyzes the hydrolysis of esters of choline, contributing to neurotransmitter metabolism and detoxification of xenobiotics. The UniProt recommended name is Butyrylcholinesterase (BCHE). This enzyme is found predominantly in plasma, liver, and nervous tissue and acts as a metabolic complement to acetylcholinesterase in cholinergic regulation.\u003cbr\u003e\u003cbr\u003eFunctionally, BCHE antibody identifies a 602-amino-acid glycoprotein secreted as a tetrameric enzyme that hydrolyzes choline esters such as butyrylcholine and succinylcholine. BCHE regulates cholinergic neurotransmission indirectly by hydrolyzing circulating esters and serves as a bioscavenger for organophosphorus compounds and nerve agents. It also participates in drug metabolism by inactivating esterified anesthetics and muscle relaxants.\u003cbr\u003e\u003cbr\u003eThe BCHE gene is located on chromosome 3q26.1-q26.2 and encodes a protein synthesized mainly in the liver before secretion into plasma. BCHE shares structural homology with acetylcholinesterase but differs in substrate specificity and inhibitor sensitivity. Its activity is regulated by genetic polymorphisms that influence catalytic efficiency and drug sensitivity. Reduced BCHE activity causes prolonged neuromuscular blockade following exposure to succinylcholine or mivacurium, a clinically relevant pharmacogenetic condition.\u003cbr\u003e\u003cbr\u003eBeyond neurotransmission, BCHE plays roles in lipid metabolism, inflammation, and neurodegenerative diseases. Elevated BCHE activity is associated with obesity and metabolic syndrome, while decreased activity has been observed in liver dysfunction, Alzheimer's disease, and chronic inflammation. Its ability to hydrolyze ghrelin and other acylated hormones links it to appetite regulation and energy balance. In neurobiology, BCHE is thought to modulate cholinergic tone during aging and cognitive decline.\u003cbr\u003e\u003cbr\u003eBCHE antibody is widely used in neurobiology, pharmacology, and metabolic disease research. It is suitable for western blotting, ELISA, and enzyme histochemistry to detect BCHE in plasma, liver, or neural tissue. This antibody supports studies of cholinesterase activity, drug metabolism, and neurodegenerative mechanisms. In toxicology, BCHE detection helps evaluate exposure to organophosphate inhibitors and nerve agents.\u003cbr\u003e\u003cbr\u003eStructurally, BCHE is a glycoprotein composed of four identical subunits stabilized by disulfide bonds and carbohydrate moieties. Its catalytic triad (Ser-His-Glu) mediates ester hydrolysis through a charge-relay mechanism.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=BCHE - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=BCHE - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=BCHE - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/BCHE - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=BCHE+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047309500781,"sku":"FY13026","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_712f6ab2-96ac-4a6d-a043-3db2a2ae7da3.jpg?v=1782237073","url":"https:\/\/www.ebiohippo.com\/products\/bche-antibody-butyrylcholinesterase-bha17135928","provider":"BioHippo","version":"1.0","type":"link"}