{"product_id":"bclaf1-antibody-bcl-2-associated-transcription-factor-1-bha17135246","title":"BCLAF1 Antibody \/ Bcl-2-associated transcription factor 1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eBCLAF1 Antibody \/ Bcl-2-associated transcription factor 1 is a anti-BCLAF1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear, cytoplasmic.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e BCLAF1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC\/IF, IP, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eThe BCLAF1 antibody targets Bcl-2-associated transcription factor 1, a nuclear protein encoded by the BCLAF1 gene that links transcriptional control with apoptosis regulation. Originally identified through interaction with anti-apoptotic Bcl-2 family members, Bcl-2-associated transcription factor 1 functions as both a transcriptional repressor and RNA-binding factor influencing mRNA processing, DNA damage responses, and chromatin organization. The BCLAF1 antibody provides researchers with a versatile reagent for studying gene regulation, cell death pathways, and the integration of nuclear signaling networks.\u003cbr\u003e\u003cbr\u003eBcl-2-associated transcription factor 1 localizes predominantly to the nucleus, where it binds chromatin and interacts with splicing and transcription complexes. It is activated by cellular stress and DNA damage, often promoting pro-apoptotic gene expression or enhancing p53-mediated transcriptional programs. The BCLAF1 antibody is used to detect nuclear foci formation following genotoxic stress, a hallmark of its activation in DNA repair pathways.\u003cbr\u003e\u003cbr\u003eBeyond apoptosis, Bcl-2-associated transcription factor 1 contributes to mRNA splicing and export. It interacts with RNA-binding proteins and components of the exon junction complex, linking transcriptional regulation to post-transcriptional RNA processing. Through these activities, it modulates the expression of genes involved in development, immune regulation, and cell differentiation. Using the BCLAF1 antibody, scientists can explore how its dual DNA- and RNA-binding capabilities coordinate nuclear responses to environmental stressors.\u003cbr\u003e\u003cbr\u003eDysregulation of BCLAF1 has been associated with cancer, viral infection, and inflammatory disease. Some viruses, such as adenovirus and cytomegalovirus, express proteins that bind and inactivate BCLAF1 to block host apoptosis and favor viral replication. Conversely, elevated expression of Bcl-2-associated transcription factor 1 has been linked to tumor suppression and enhanced sensitivity to chemotherapeutic agents. These opposing effects underscore the complexity of its biological roles. The BCLAF1 antibody supports functional studies designed to dissect these mechanisms in both normal and transformed cells.\u003cbr\u003e\u003cbr\u003eThe BCLAF1 antibody is applicable in western blotting, immunofluorescence, chromatin immunoprecipitation, and immunohistochemistry. It provides high specificity for the nuclear form of the protein and allows visualization of expression across tissue types. Investigators studying DNA damage signaling, RNA metabolism, or apoptosis can utilize this antibody to identify dynamic changes in protein localization and abundance following genotoxic or apoptotic stimuli.\u003cbr\u003e\u003cbr\u003e\n\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=BCLAF1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=BCLAF1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=BCLAF1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/BCLAF1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=BCLAF1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047285678445,"sku":"FY12343","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_7de368cd-c43c-4e35-8d2d-ee1937dd146f.jpg?v=1782236994","url":"https:\/\/www.ebiohippo.com\/products\/bclaf1-antibody-bcl-2-associated-transcription-factor-1-bha17135246","provider":"BioHippo","version":"1.0","type":"link"}