{"product_id":"bclaf1-antibody-bcl2-associated-transcription-factor-1-btf-bha17136257","title":"BCLAF1 Antibody \/ BCL2-associated transcription factor 1 \/ BTF","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eBCLAF1 Antibody \/ BCL2-associated transcription factor 1 \/ BTF is a anti-BCLAF1 Rabbit antibody Recombinant Rabbit Monoclonal clone 32B81 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF) with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e BCLAF1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 32B81, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC, IF\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eBCLAF1 antibody detects BCL2-associated transcription factor 1, encoded by the BCLAF1 gene. BCL2-associated transcription factor 1 is a nuclear protein originally identified through its binding to anti-apoptotic members of the Bcl-2 family. BCLAF1 antibody provides researchers with a highly specific reagent to study apoptosis, transcriptional regulation, and cellular stress responses.\u003cbr\u003e\u003cbr\u003eBCL2-associated transcription factor 1 contains multiple functional domains that enable it to interact with transcriptional regulators, splicing machinery, and apoptotic signaling proteins. Research using BCLAF1 antibody has shown that it can act as a transcriptional repressor, influencing gene networks associated with cell death and survival. Its ability to interact with Bcl-2 family proteins positions it at the intersection of transcription and apoptosis regulation.\u003cbr\u003e\u003cbr\u003eStudies with BCLAF1 antibody have demonstrated that the protein also functions in RNA processing. BCL2-associated transcription factor 1 associates with splicing factors and contributes to pre-mRNA processing. This activity provides an additional layer of gene expression control, ensuring that transcription and splicing are coordinated in response to cellular needs.\u003cbr\u003e\u003cbr\u003eBeyond transcription and splicing, BCLAF1 influences DNA damage responses. Research using BCLAF1 antibody has revealed that the protein participates in DNA repair pathways and helps maintain genomic stability. Its expression is upregulated in response to genotoxic stress, and it contributes to apoptosis when DNA damage is severe. This dual role in repair and apoptosis underscores its importance in genome maintenance.\u003cbr\u003e\u003cbr\u003eDysregulation of BCLAF1 has been linked to cancer and immune disorders. Overexpression can enhance apoptosis and impair cell survival, while reduced expression contributes to resistance to cell death. Studies with BCLAF1 antibody have also associated mutations and altered expression with autoimmune diseases. These findings demonstrate that balanced regulation of BCL2-associated transcription factor 1 is essential for normal physiology.\u003cbr\u003e\u003cbr\u003eBCLAF1 antibody is widely applied in western blotting, immunohistochemistry, and immunofluorescence. Western blotting detects full-length protein and isoforms, immunohistochemistry identifies nuclear expression patterns in tissues, and immunofluorescence reveals its nuclear localization and distribution within subnuclear domains. These applications make BCLAF1 antibody valuable for research into transcription, apoptosis, and DNA repair.\u003cbr\u003e\u003cbr\u003eBy supplying validated BCLAF1 antibody reagents,\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=BCLAF1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=BCLAF1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=BCLAF1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/BCLAF1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=BCLAF1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047323525485,"sku":"FY13355","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_2ded635e-7569-4734-af3b-503ed73993bf.jpg?v=1772019430","url":"https:\/\/www.ebiohippo.com\/products\/bclaf1-antibody-bcl2-associated-transcription-factor-1-btf-bha17136257","provider":"BioHippo","version":"1.0","type":"link"}