{"product_id":"beaverbeads-magrose-cooh-carboxyl-magnetic-agarose-beads-bhd13200041","title":"BeaverBeads Magrose COOH Carboxyl Magnetic Agarose Beads","description":"\u003ch2\u003eProduct Overview\u003c\/h2\u003e\u003cp\u003eBeaverBeads™ Magrose COOH are superparamagnetic microspheres built on an agarose shell around an Fe\u003csub\u003e3\u003c\/sub\u003eO\u003csub\u003e4\u003c\/sub\u003e core, presenting a high density of surface carboxyl groups. The porous agarose matrix gives a large accessible surface without the non-specific adsorption typical of bare polymer surfaces, and the superparamagnetic core allows the beads to be collected in seconds on a magnetic separator and fully redispersed when the field is removed.\u003c\/p\u003e\u003cp\u003eCarboxyl groups are the most widely used handle for covalent immobilisation. Activated with EDC and NHS, they form stable amide bonds with any ligand carrying a primary amine — antibodies, proteins, peptides, amine-modified oligonucleotides and small molecules. Once coupled, the beads become a custom affinity support for the target of your choice.\u003c\/p\u003e\u003ch2\u003eHow it works\u003c\/h2\u003e\u003cp\u003eThe published coupling route washes the beads into MES buffer at pH 5.0, activates the surface carboxyls with fresh EDC and NHS for 30 minutes at 25 °C, then couples 50–200 µg of ligand at pH 8.0 for 2 hours at 25 °C (or 1 hour at 25 °C followed by overnight incubation at 4 °C). Residual activated esters are quenched and the conjugate is stored in preservative buffer at 4 °C. The full procedure is in the user manual linked under Documents.\u003c\/p\u003e\u003ch2\u003eApplications\u003c\/h2\u003e\u003cp\u003eImmunoprecipitation and co-immunoprecipitation with a covalently coupled capture antibody; affinity purification of proteins, peptides and oligonucleotides; chemiluminescent immunoassay development; cell sorting; phage display screening; and construction of custom affinity supports for drug-molecule capture.\u003c\/p\u003e\u003ch2\u003eCompatibility notes\u003c\/h2\u003e\u003cp\u003eMagrose COOH is the large-particle, agarose-matrix member of the BeaverBeads COOH family. If your assay needs a monodisperse sub-micron or micron polymer bead instead — for example for automated liquid handling or flow-based detection — the Mag COOH series (300 nm, 1 µm, 2 µm, 5 µm) covers that range. Beads are compatible with manual magnetic separators and with automated magnetic-rod and plate-based workstations.\u003c\/p\u003e\u003ch2\u003eHandling and safety\u003c\/h2\u003e\u003cp\u003e\u003cstrong\u003eDo not freeze, dry out or centrifuge these beads\u003c\/strong\u003e — all three cause irreversible aggregation and permanent loss of surface activity. Vortex or sonicate briefly to a uniform suspension before every use. A magnetic separator is required. Keep the product away from metal objects and electronic devices; superparamagnetic material and magnetic separators should be kept well clear of implanted electronic devices such as cardiac pacemakers.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eFor Research Use Only.\u003c\/strong\u003e Not for use in diagnostic or therapeutic procedures. Specifications are those published by the manufacturer, BEAVER Biomedical Engineering Co., Ltd.\u003c\/p\u003e","brand":"Beaver Biomedical Engineering Co Ltd","offers":[{"title":"5 mL","offer_id":53718873702765,"sku":"70103-5","price":580.0,"currency_code":"USD","in_stock":true},{"title":"50 mL","offer_id":53718873735533,"sku":"70103-50","price":2960.0,"currency_code":"USD","in_stock":true},{"title":"Other size - enquire","offer_id":53718873768301,"sku":"70103","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/beaverbeads-magrose-cooh-70103-beads.png?v=1786561284","url":"https:\/\/www.ebiohippo.com\/products\/beaverbeads-magrose-cooh-carboxyl-magnetic-agarose-beads-bhd13200041","provider":"BioHippo","version":"1.0","type":"link"}