{"product_id":"beaverbeads-magrose-nh2-amino-magnetic-agarose-beads-bhd13200047","title":"BeaverBeads Magrose NH2 Amino Magnetic Agarose Beads","description":"\u003ch2\u003eProduct Overview\u003c\/h2\u003e\u003cp\u003eBeaverBeads™ Magrose NH\u003csub\u003e2\u003c\/sub\u003e are superparamagnetic microspheres with an Fe\u003csub\u003e3\u003c\/sub\u003eO\u003csub\u003e4\u003c\/sub\u003e core and an agarose shell carrying free primary amino groups on the surface. Amino beads are the complement to carboxyl beads: where COOH beads couple to amines on your ligand, NH\u003csub\u003e2\u003c\/sub\u003e beads couple to carboxyl groups, aldehydes or other amine-reactive chemistries on the ligand — useful when the ligand's own amines are in the binding site and must be left alone.\u003c\/p\u003e\u003cp\u003eThe agarose matrix is porous and hydrophilic, which gives more accessible surface per bead and markedly lower non-specific protein adsorption than a bare polymer surface. Magnetic response is fast enough that separation takes seconds on a standard separator.\u003c\/p\u003e\u003ch2\u003eSpecification\u003c\/h2\u003e\u003cp\u003eMean particle size 30–150 µm. Surface amino content approximately 50 µmol per mL of gel. Saturation magnetisation 41.09 emu\/g. Supplied as a 20% (v\/v) suspension. Shelf life 2 years at 2–8 °C.\u003c\/p\u003e\u003ch2\u003eApplications\u003c\/h2\u003e\u003cp\u003eEnrichment, separation and purification of biomolecules — proteins, peptides, oligonucleotides and drug molecules. Typical uses are building custom affinity supports by coupling a carboxyl-bearing ligand, glutaraldehyde-mediated immobilisation of proteins, immunoprecipitation with a covalently attached capture antibody, and as an intermediate surface for further chemical modification.\u003c\/p\u003e\u003ch2\u003eChoosing between Magrose surfaces\u003c\/h2\u003e\u003cp\u003eWithin the BeaverBeads Magrose family: COOH (Cat. 70103) for coupling to ligand amines with EDC\/NHS; NH\u003csub\u003e2\u003c\/sub\u003e (this product, Cat. 70203) for coupling to ligand carboxyls or through glutaraldehyde; NHS (Cat. 70702) already activated, for direct amine coupling with no activation step; OH (Cat. 70802) as an unfunctionalised starting surface. All share the same 30–150 µm magnetic agarose matrix.\u003c\/p\u003e\u003ch2\u003eHandling and safety\u003c\/h2\u003e\u003cp\u003e\u003cstrong\u003eDo not freeze, dry out or centrifuge the beads\u003c\/strong\u003e — each causes irreversible aggregation and loss of surface activity. Vortex or sonicate briefly to an even suspension before every use. A magnetic separator is required. Keep beads and separators away from metal objects, electronic devices and implanted electronic devices such as cardiac pacemakers.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eFor Research Use Only.\u003c\/strong\u003e Not for use in diagnostic or therapeutic procedures. Specifications are those published by the manufacturer, BEAVER Biomedical Engineering Co., Ltd.\u003c\/p\u003e","brand":"Beaver Biomedical Engineering Co Ltd","offers":[{"title":"5 mL","offer_id":53719332421997,"sku":"70203-5","price":580.0,"currency_code":"USD","in_stock":true},{"title":"50 mL","offer_id":53719332454765,"sku":"70203-50","price":2960.0,"currency_code":"USD","in_stock":true},{"title":"Other size - enquire","offer_id":53719332487533,"sku":"70203","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/ps35877620-agarose_amino_magnetic_beads_for_protein_purification_30_150_m_5_ml.jpg?v=1786566310","url":"https:\/\/www.ebiohippo.com\/products\/beaverbeads-magrose-nh2-amino-magnetic-agarose-beads-bhd13200047","provider":"BioHippo","version":"1.0","type":"link"}