{"product_id":"beaverbeads-stool-dna-kit-bht13200004","title":"BeaverBeads Stool DNA Kit","description":"\u003ch2\u003eProduct Overview\u003c\/h2\u003e\u003cp\u003eThe BeaverBeads™ Stool DNA Kit extracts genomic DNA from stool using superparamagnetic silica beads and pre-made buffers, recovering both host DNA and the DNA of the intestinal flora. Stool is one of the hardest matrices in molecular biology — it is rich in bile salts, polysaccharides and humic substances that carry through conventional preps and inhibit polymerases downstream — so the value of the kit is in the buffer chemistry rather than the bead alone.\u003c\/p\u003e\u003cp\u003eThe method uses no phenol and no chloroform. That removes a fume-hood requirement and a hazardous-waste stream from a protocol that is often run at high sample numbers.\u003c\/p\u003e\u003ch2\u003ePerformance\u003c\/h2\u003e\u003cp\u003eYield is reported at \u003cstrong\u003eover 2 µg from a 200 mg sample\u003c\/strong\u003e. Recovery is stated at 40–70% depending on the sample — an honest range, because stool composition varies enormously between donors and diets. The manufacturer reports consistent Ct values across repeat extractions, and quantifies output on a Qubit fluorometer. Purified DNA is suitable for PCR amplification, sequencing and detection.\u003c\/p\u003e\u003ch2\u003eFormats\u003c\/h2\u003e\u003cp\u003eTwo configurations are listed. The 100-reaction pack is the standard tube-format kit. The 32-reaction pack is supplied as a \u003cstrong\u003eU-bottom pre-filled plate\u003c\/strong\u003e, where the buffers are already dispensed into the plate — intended for automated magnetic extraction platforms, where it removes the buffer-dispensing steps and the pipetting error that comes with them. Both run the same chemistry; choose by whether you work manually or on an instrument.\u003c\/p\u003e\u003ch2\u003eApplications\u003c\/h2\u003e\u003cp\u003eGut microbiome profiling by 16S rRNA amplicon sequencing or shotgun metagenomics; detection of enteric pathogens by PCR and qPCR; host genomic DNA recovery from stool for colorectal cancer screening research; and any downstream assay that needs inhibitor-free DNA from a faecal matrix.\u003c\/p\u003e\u003ch2\u003eHandling and safety\u003c\/h2\u003e\u003cp\u003eFollow the wash and drying steps carefully. Residual ethanol carried into the eluate is the most common cause of failed downstream reactions with any magnetic-bead nucleic acid prep. Do not freeze the magnetic beads. A magnetic separator is required.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eBiosafety note:\u003c\/strong\u003e stool is a potentially infectious material. Handle at the containment level your institution requires for human faecal samples, and treat all waste accordingly.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eFor Research Use Only.\u003c\/strong\u003e Not for use in diagnostic or therapeutic procedures. Specifications are those published by the manufacturer, BEAVER Biomedical Engineering Co., Ltd. A downloadable user manual for this catalogue number was not published at the time of listing; contact us if you need one before ordering.\u003c\/p\u003e","brand":"Beaver Biomedical Engineering Co Ltd","offers":[{"title":"100 reactions (tube format)","offer_id":53719334158701,"sku":"70411-100","price":2580.0,"currency_code":"USD","in_stock":true},{"title":"32 reactions (U-bottom pre-filled plate)","offer_id":53719334191469,"sku":"70411-32U","price":1018.0,"currency_code":"USD","in_stock":true},{"title":"Other size - enquire","offer_id":53719334224237,"sku":"70411","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/beaverbeads-stool-dna-kit-70411-product.png?v=1786565732","url":"https:\/\/www.ebiohippo.com\/products\/beaverbeads-stool-dna-kit-bht13200004","provider":"BioHippo","version":"1.0","type":"link"}