{"product_id":"bgn-antibody-biglycan-bha17136100","title":"BGN Antibody \/ Biglycan","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eBGN Antibody \/ Biglycan is a anti-BGN Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC) with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e BGN\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eBGN antibody detects Biglycan, a small leucine-rich proteoglycan (SLRP) that regulates extracellular matrix assembly, collagen fibrillogenesis, and innate immune signaling. The UniProt recommended name is Biglycan (BGN). This extracellular matrix component binds collagens, growth factors, and cell surface receptors to coordinate tissue structure and repair responses.\u003cbr\u003e\u003cbr\u003eFunctionally, BGN antibody identifies a 368-amino-acid core protein decorated with chondroitin\/dermatan sulfate glycosaminoglycan chains. Biglycan interacts with collagen types I, II, and VI to stabilize fibril formation and maintain matrix integrity. It also binds toll-like receptors TLR2 and TLR4, activating NF-kappaB-mediated inflammatory signaling in macrophages and fibroblasts. Through its dual structural and signaling roles, Biglycan serves as a molecular bridge between tissue mechanics and innate immune pathways.\u003cbr\u003e\u003cbr\u003eThe BGN gene is located on chromosome Xq28 and is expressed in connective tissues such as bone, tendon, skin, and vascular walls. It is particularly abundant in mineralized tissues, where it regulates osteoblast differentiation, bone mineralization, and matrix maturation. BGN expression is dynamically regulated during wound healing, inflammation, and fibrosis, contributing to tissue remodeling and repair.\u003cbr\u003e\u003cbr\u003ePathologically, altered BGN levels have been associated with muscular dystrophy, atherosclerosis, osteogenesis imperfecta, and cancer. Elevated Biglycan expression promotes fibrosis and chronic inflammation by sustaining macrophage activation, while deficiency leads to impaired matrix organization and skeletal fragility. Research using BGN antibody supports studies in extracellular matrix biology, inflammation, and connective tissue disease mechanisms.\u003cbr\u003e\u003cbr\u003eBGN antibody is validated for western blotting, immunohistochemistry, and ELISA to detect extracellular matrix proteoglycans. \u003cbr\u003e\u003cbr\u003eStructurally, Biglycan contains tandem leucine-rich repeat (LRR) motifs that mediate protein-protein interactions and a signal peptide directing secretion to the extracellular matrix. The glycosaminoglycan attachment sites near its N-terminus allow Biglycan to form proteoglycan complexes essential for biomechanical strength and cell signaling. This antibody aids in the analysis of BGN's structural role in connective tissue maintenance and its signaling function in inflammation and repair.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=BGN - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=BGN - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=BGN - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/BGN - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=BGN+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047317889389,"sku":"FY13198","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_4a8a614f-24d0-4537-a3f1-f9f7dd657053.jpg?v=1782237095","url":"https:\/\/www.ebiohippo.com\/products\/bgn-antibody-biglycan-bha17136100","provider":"BioHippo","version":"1.0","type":"link"}