{"product_id":"bilobalide-bhb21300057","title":"Bilobalide","description":"\u003ch3\u003eProduct details\u003c\/h3\u003e \u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eChemical name:\u003c\/strong\u003e (5aR- (3aS*,5aα,8b,8aS*,9a,10aα))- 9-(1,1-dimethylethyl)- 10,10a-dihydro- 8,9-dihydroxy- 4H,5aH,9H-furo[2,3-b]furo[3',2':2,3]cyclopenta[1,2-c]furan- 2,4,7(3H,8H)-trione.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCAS number:\u003c\/strong\u003e 33570-04-6\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMolecular formula:\u003c\/strong\u003e C15H18O8.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurity:\u003c\/strong\u003e \u0026gt;99.5%\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eConcentration:\u003c\/strong\u003e 1-100 µM.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eForm:\u003c\/strong\u003e Lyophilized Powder\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSolubility:\u003c\/strong\u003e 100 mM in DMSO. Centrifuge all product preparations before use (10000 x g 5 min).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReconstitution:\u003c\/strong\u003e Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in sterile water at a concentration of at least 0.1 mg\/mL. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. It is recommended to prepare fresh solutions in working buffers just before use. For long-term storage of diluted solutions, we recommend adding 0.1% BSA. Repeat freeze-thawing may result in loss of activity\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eShipping:\u003c\/strong\u003e Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e 5-HT3A, GABA(A) receptors\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSource:\u003c\/strong\u003e Natural\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eActivity:\u003c\/strong\u003e Bilobalide is a 5-HT3A1 and GABAA2 channel blocker.\u003c\/li\u003e\n\u003c\/ul\u003e \u003ch3\u003eScientific background\u003c\/h3\u003e \u003cp\u003eSerotonin (5-hydroxytryptamine, 5-HT) is a monoamine neurotransmitter synthesized from the amino acid tryptophan by the enzyme tryptophan-decarboxylase. The 5-HT3 channel is a ligand-gated ion channel receptor of the Cys-loop channel family1.The GABA(A) receptor is also a ligand-gated ion channel. It is the major receptor type for the GABA inhibitory neurotransmitter in the central nervous system2.Bilobalide is a natural non-selective blocker of the 5-HT3A and GABA(A) channels. Bilobalide is a sesquiterpene trilactone extracted from the leaves of the tree Ginkgo biloba. Bilobalide has an effective concentration of 1-100 μM and an IC50 of 470 μM for the 5-HT3A receptor3 and an IC50 of 4.6±0.5 μM for the GABA(A) receptor. Bilobalide also binds to other anion Cys-loop receptors such as glycine receptors with a much higher affinity than to the 5-HT3A receptor.Bilobalide inhibits direct GABA mediated currents from recombinant human GABA(A) receptors thus suggesting it enhances neuronal excitability. Bilobalide is used in traditional Japanese medicine as a memory enhancer, treating symptoms associated with mild-moderate dementia and improving cognitive functions related to old age and senility3.\u003c\/p\u003e \u003cp\u003e\u003cstrong\u003eLead time:\u003c\/strong\u003e 1-2 Business Days\u003c\/p\u003e \u003cp\u003e\u003cstrong\u003eCountry of origin:\u003c\/strong\u003e Israel\/IL\u003c\/p\u003e \u003ch3\u003eApplications key\u003c\/h3\u003e \u003cp\u003eApplication key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.\u003c\/p\u003e \u003cp\u003e\u003cstrong\u003eBioassay tested:\u003c\/strong\u003e Yes\u003c\/p\u003e","brand":"Alomone Labs","offers":[{"title":"10 mg \/ 1","offer_id":53249707213165,"sku":"B-145-10MG-1","price":66.0,"currency_code":"USD","in_stock":true},{"title":"25 mg \/ 1","offer_id":53311555567981,"sku":"B-145-25MG-1","price":145.0,"currency_code":"USD","in_stock":true},{"title":"50 mg \/ 1","offer_id":53311555600749,"sku":"B-145-50MG-1","price":262.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/B-145_gr.gif?v=1778544413","url":"https:\/\/www.ebiohippo.com\/products\/bilobalide-bhb21300057","provider":"BioHippo","version":"1.0","type":"link"}